Summary. Prostaglandins (El, E 2 and F2~ ) stimulated the chloride transport of the frog corneal epithelium with maximal effects at 10 -5 M in the aqueous side. This stimulation does not occur in Cl-free solutions and the net 36C1 flux increased proportionally to the short-circuit current. Polyphloretin phosphate (PPP) and diphloretin phosphate (DPP) inhibited the response if added within 3 rain before PGE 1. The maximal response to epinephrine 10-SM and dibutyryl cyclic AMP 10-3M was not changed by further addition of prostaglandins, but these drugs produced their full effect when administered at the peak of the response of prostaglandins. The maximal response to theophylline 10 -5 M was increased by PGE 1. PPP and DPP did not modify the response to epinephrine. Prostaglandin stimulation of the chloride transport was accompanied by increased light transmission through partially opaque corneas. The known release of prostaglandins in the aqueous humor can be associated to a direct action on the corneal epithelium manifested in the activation described herein.The transparency of the cornea is dependent on the state of hydration of the stroma. The two principal cell layers of this tissue, the epithelium in front, and the endothelium inside, contribute by their permeability properties and, by the presence of ionic pumps, to the control of the state of hydration of the stroma (Maurice, 1969;.The epithelium of the frog cornea transports chloride ions out into the tear side (Zadunaisky, 1966), and this mechanism has been shown to be
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