The stimulation of chloride transport by prostaglandins and their interaction with epinephrine, theophylline, and cyclic AMP in the corneal epithelium

Beitch, Barbara R.; Beitch, Irwin; Zadunaisky, J. A.

doi:10.1007/bf01869987

Cited by 44 publications

(16 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To our knowledge the drugs we used to enhance the wound-generated EF altered only the TCPD and did so by different mechanisms. PGE2 enhances chloride efflux, aminophylline and ascorbic acid inhibit phospodiesterase breakdown of cAMP, which also enhances Cl -efflux ( ., 1974;Buck and Zadunaisky, 1975) and AgNO3 increases both early Na + uptake and later Cl -efflux (Klyce and Marshall, 1982). Similarly, we chose drugs that suppressed the woundgenerated EF, by reducing the TCPD in different ways.…”

Section: Discussionmentioning

confidence: 99%

Nerve regeneration and wound healing are stimulated and directed by an endogenous electrical field in vivo

Song

Zhao

Forrester

et al. 2004

Journal of Cell Science

153

112

View full text Add to dashboard Cite

Biological roles for naturally occurring, extracellular physiological electric fields have been proposed over the past century. However, in the molecular era, many biologists presume that electric fields have little physiological relevance because there has been no unequivocal demonstration of their importance at the single-cell level in vivo. We have used an in vivo rat corneal model, which generates its own endogenous electric field and show that nerve sprouting, the direction of nerve growth and the rate of epithelial wound healing are controlled coordinately by the wound-induced electric field.

show abstract

Section: Discussionmentioning

confidence: 99%

Nerve regeneration and wound healing are stimulated and directed by an endogenous electrical field in vivo

Song

Zhao

Forrester

et al. 2004

Journal of Cell Science

153

112

View full text Add to dashboard Cite

show abstract

“…rabbit ileum, rabbit gall-bladder (Frizzell, Dugas & Schultz, 1975), rat colon (Binder & Rawlins, 1973) 60-90 Q cm2, guinea-pig gallbladder (Saverymuttu & Wood, 1977), and fly salivary gland (Prince & Berridge, 1973) to secretagogues differs from the response of a number of tight epithelia, e.g. rabbit colon (Frizzell, Koch & Schultz, 1976) 220-270 Q cm2, guinea-pig seminal vesicle (Levine, Rinaldo & Schultz, 1976), rabbit cornea (Beitch, Beitch & Zadunaisky, 1974), and frog skin (Cuthbert & Painter, 1968). In the latter group of tight epithelia, the secretagogues have no inhibitory effect on either net or unidirectional transepithelial Na+ fluxes, indeed they may stimulate Na+ absorption in frog skin, whilst causing marked increases in the short-circuit current due to stimulated Cl-secretion.…”

Section: Discussionmentioning

confidence: 99%

The effects of theophylline and choleragen on sodium and chloride ion movements within isolated rabbit ileum.

Naftalin¹,

Simmons²

1979

The Journal of Physiology

View full text Add to dashboard Cite

SUMMARY1. Theophylline (10 mM) and choleragen change the direction of net Cl-movements across rabbit ileum, in the short-circuit current condition, from absorption to secretion. The specific activity ratio R of C1-tracers within the tissue coming from mucosal and serosal solutions respectively is increased, which is consistent with an increase in Cl-exchange flux across the mucosal border.2. Net Na+ movement is also changed from net absorption to secretion by theophylline and choleragen; the specific activity ratio R of Na+ tracers is raised by theophylline. Because of the large paracellular component to transepithelial Na+ movements, an increase in Na+ exchange flux across the mucosal border is not detected.3. 2,4,6-Triaminopyrimidine (20 mM) which has been previously shown to block paracellular Na+ movements, blocks both the theophylline and choleragen-dependent reversal of net Na+ movement by preventing the decrease in m-s Na flux. The theophylline-dependent increase in the ratio R of Na+ is still present, and is consistent with an increase in Na+ exchange flux across the mucosal border -unmasked by removal of the paracellular flux components. 4. Ouabain (0.1 mM) abolishes net absorption of Na+ and Cl-in control and net secretion of Na+ and Cl-in theophylline-treated tissue. Ouabain does not affect the theophylline-dependent increase in Cl-exchange across the mucosal border.5. Replacement of Ringer Cl-with SO'-or Na+ by choline prevents the effects of theophylline and choleragen on Na+ and C1-fluxes respectively.6. Ethacrynate (0 1 mM) prevents the theophylline-dependent effects on net Na+ movement. Raising ethacrynate to 0-2 mm abolishes the effects of theophylline on Cl-exchange.An interpretation of these results is that theophylline and choleragen raise the Cl-permeability of the brush border. This increases NaCl leakage from the hypertonic lateral intercellular space into the mucosal solution thereby causing secretion. The selective action of triaminopyrimidine and ethacrynate (0.1 mM) on Na+ flux indicates that Na+ and Cl-move via separate transport pathways across the mucosal border.

show abstract

“…It should be noted that a decreased transepithelial conductance is not the invariable response of all epithelia following exposure to secretagogues. Frizzell, Koch & Schultz (1976) with rabbit colon and Beitch, Beitch & Zadunaisky (1974) with rabbit cornea have shown that cyclic AMP increases active Cl-secretion, short circuit current and also transepithelial conductance in both these 'tight' epithelia. More recently Frizzell (1977) has shown that Ca2+ ionophore A 23187 causes increased short-circuit current due to active Cl-secretion, accompanied by increased electrical conductance across rabbit colon; whereas Bolton & Field (1977) have shown that A 23187 increases short circuit current, and Cl-secretion but decreases transepithelial conductance across rabbit ileum.…”

Section: Introductionmentioning

confidence: 97%

Electrophysiological and electron‐microscopical correlations with fluid and electrolyte secretion in rabbit ileum.

Holman¹,

Naftalin²,

Simmons³

et al. 1979

The Journal of Physiology

View full text Add to dashboard Cite

SUMMARY1. The effects of theophylline and triaminopyrimidine on the passive permeability of Na+ and Cl-across sheets of rabbit ileum treated with 0-1 mM-ouabain were examined by determining the NaCl: mannitol dilution potentials, K+: Na+; choline: Na+ and S02-: Cl-biionic potentials. The results indicate (a) that triaminopyrimidine reduces paracellular Na+ and K+ permeability without affecting Cl-permeability and (b) that theophylline increases Cl-permeability without affecting Na+ permeability and (c) that neither theophylline nor triaminopyrimidine interfere with each other's action. This is further evidence consistent with separate routes for paracellular Na+ and Cl-movement. 2. Electrical resistance changes across sheets of actively transporting rabbit ileum were measured as a function of time in various conditions. Theophylline has a biphasic effect on resistance. Initially it decreases resistance from 56 Q cm2 (control) to 40 Q cm2. In the ensuing 30 min, resistance rises to 50 Q cm2; whereas it falls to 30 Q cm2 in controls.When theophylline is present, with triaminopyrimidine, or with galactose, no secondary rise in tissue resistance occurs. The initial decrease in resistance is consistent with a theophylline-dependent increase in Cl-conductance and the secondary rise in resistance may be attributed to collapse of the lateral intercellular space following leakage of NaCl and fluid into the mucosal solution.3. Electron microscopy of glutaraldehyde-fixed tissue confirms the above views, since, with theophylline present, the lateral intercellular spaces are collapsed and with both triaminopyrimidine and theophylline, or both theophylline and 20 mMgalactose present the spaces remain open.4. It is shown in the Discussion that the theophylline-or choleragen-induced increase in passive Cl-permeability of the mucosal border is the only requirement necessary to explain the increase in electrogenic Cl-secretion, the increase in shortcircuit current, as well as neutral secretion of NaCl and net fluid secretion.

show abstract

The stimulation of chloride transport by prostaglandins and their interaction with epinephrine, theophylline, and cyclic AMP in the corneal epithelium

Cited by 44 publications

References 21 publications

Nerve regeneration and wound healing are stimulated and directed by an endogenous electrical field in vivo

Nerve regeneration and wound healing are stimulated and directed by an endogenous electrical field in vivo

The effects of theophylline and choleragen on sodium and chloride ion movements within isolated rabbit ileum.

Electrophysiological and electron‐microscopical correlations with fluid and electrolyte secretion in rabbit ileum.

Contact Info

Product

Resources

About