Black locust (Robinia pseudoacacia) is recognised as a forest species of interest due to its multiple uses. The management of forest genetic resources and their efficient conservation suffer from variations in traits and start with seed germination. The aim of the current study was to investigate the germination of seeds obtained from plus trees selected in eight Romanian provenances, as well as to investigate the influence of the origin upon plants’ growth and development. Two experiments were undertaken to test seed germination: one treatment involved water-soaked seeds and heat/cold treatment, while the other treatment was based on sulphuric acid, at different concentrations (50, 70, 90%). The results were correlated with the morphological analysis of the seeds. Satu-Mare had the lowest germination rate within both treatments. Sulphuric acid did not improve seed germination as much as the heat treatment. The highest germination rate occurred for the water and temperature treatment on seeds from Bihor provenance (68.2%). The most distant provenance was Bihor, in inverse correlation with Bistrița Năsăud and grouped separately within the hierarchical dendrogram of cluster analysis based on the analysed parameters of the provenances investigated. The results demonstrated that the genotypes and environmental heterogeneity of the seed origin within the provenances may finally result in different performances.
Black locust (Robinia pseudoacacia L.) was among the first North-American tree species imported in Europe. In Romania, black locust has established itself as a forest tree appreciated for multiple uses. The objective of the hereby study was to identify a quality planting material at black locust using seeds from trees with superior traits from five stands geographically close, located in North-western of Romania. An empirical selection was done, thus trees with the most favourable traits were selected as plus trees. Among the averages of the main traits (tree height, diameter at breast height, basal area, self pruning trunk length, crown diameter) of the plus trees from the five stands, there were registered significant differences, and two stands stood out with a high biomass growth. Even if the stands had different ages (between 20-35 year), the age did not influence significantly the growth traits of the trees. The seeds of the plus trees (open-pollinated) from all the stands had large size (mean seed weight of 0.057 g/seed). The seedling emergence rate was high, especially in the solarium condition (between 52.7-73.7% compared with 33.0-41.3% in the field). Coefficient of genetic correlation and heritability calculated for the seedlings belongings to half-sib families highlighted that black locust breeding can be extremely effective by a proper selection.
The current study aimed to investigate the effect of different treatments applied to sessile oak seeds in order to improve the germination features, development and growth of seedlings. The seeds were subjected to electric field, electromagnetic field and γ rays’ treatments. For the electric field study three distinct voltages (10V, 30V and 50V) and three values of exposure time (15 min., 35 min. and 60 min.) were considered. Displaying the seeds in an electric field for 60 minutes increased the germination rate with 58.89%. The highest value for the seedling’s height was obtained when seeds were exposed for 15 minutes at an intensity of 30V. The interaction between the intensity and the timing showed that treating seeds with 30V for 35 minutes improved significantly the seedlings peculiarities. In regard to the electromagnetic field, the seeds were subjected to a single value of electromagnetic field (B=0,4mT; i=0,680 A) and three values of exposure time: 10 min., 20 min. and 30 min. The sessile oak seeds subjected to the electromagnetic field for 20 minutes induced the highest value of germination percentage (90.00%) and germination index (1.81) and those seeds produced plantlets with high diameter (2.69 mm) and with significant differences towards non-treated plants. For γ rays treatments a dosage of 2 Gy induced the highest value of germination percentage (86.67%), of germination index (2.02); of germination energy (7.7); and of germination speed (7.24). A dosage of 6 Gy decreased the values of the same parameters. Our results showed that a dosage of 2 Gy improved also the growth parameters such as plantlets diameter (2.65 mm) and height (11.25 cm). In short, the physical treatments represent an ecological substitute approach towards the chemical substances presently used to improve seeds germination. Using physical treatments for seeds germination and growth of seedlings might be a harmless alternative for environment.
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