Male fertility has become a very discussed topic among researchers in the last years. With the latest environmental changes, use of genetically modified foods, lack of physical exercise, work overload and frequent burnouts, the number of men prone to infertility is continuously increasing. In this study, we aimed to evaluate the influence of age on the sperm quality in presumably infertile males. In this purpose, we have investigated a significant number of semen samples, by using macroscopic and microscopic standard evaluation methods to determine the volume, viscosity, density, morphology, motility and vitality of spermatozoa. Our data suggest that spermatozoa viability and progressive motility, two of the most important indicators of sperm quality, decrease with age, suggesting the role of patient's age in male infertility, as well as the necessity for a better understanding of the intimate mechanisms and risks involved, as an increasing number of men are choosing to delay fatherhood.