Rivers in the KPK Peshawar region of Pak are subjected to a variety of anthropogenic impacts, Agricultural operations, industry, and urban garbage are all examples. The purpose of The goal of this research was to find out the degree of water contamination by showing a testing that includes in situ bio-monitoring of water constituents on Geno-toxicity in local fish species. Three places in the region were tested for Geno-toxicity using the micronucleus test. At sample locations like the urban perimeter, farmland, and the fertiliser industry, it was interesting to observe if there were any differences between monitored species control and such Characidium fasciatum, Astyanax fasciatus, Astyanax altiparanae. Records showed that the species from sites one, Two, and Three had a considerably greater frequency of Micronucleus when compared to fish from site Four. The frequency of Micronucleus was substantially higher in and A. altiparanae and A. fasciatus erythrocytes at sites one and two. Micronucleus frequencies were higher at site three in C. fasciatum. Micronucleus induction in C. fasciatum was associated to Cr levels in water and sediment, whereas A. altiparanae and A. fasciatum were linked to zinc levels in water and sediment. Received: 11 July 2022 / Accepted: 19 October 2022 / Published: 5 November 2022
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