Plant phenolics and flavonoids have a powerful biological activity, which outlines the necessity of their determination. The phenolics and flavonoids content of 20 medicinal plants were determined in the present investigation. The phenolic content was determined by using Folin-Ciocalteu assay. The total flavonoids were measured spectrophotometrically by using the aluminium chloride colorimetric assay. The results showed that the family Mimosaceae is the richest source of phenolics, (Acacia nilotica: 80.63 mg gallic acid equivalents, Acacia catechu 78.12 mg gallic acid equivalents, Albizia lebbeck 66.23 mg gallic acid equivalents). The highest total flavonoid content was revealed in Senna tora which belongs to the family Caesalpiniaceae. The present study also shows the ratio of flavonoids to the phenolics in each sample for their specificity.
Two new flavonoids named castilliferol 1 and castillicetin 2, as well as a known compound, isochlorogenic acid 3, were isolated from the whole plant of Centella asiatica. Isolates 1 and 2 exhibited good antioxidant activity using 2,2-diphenyl-1-picryl hydrazyl radical solution with IC(50) values of 23.10 and 13.30, respectively. The structures of these isolates were determined by analytical and spectral data, including 1-D and 2-D NMR spectra.
Background:Family Zingiberaceae consists of the large number of medicinal plants and is well-known for its use in ethnomedicine and play a major role in Indian System of Medicine, Ayurveda.Objective:The aim of this study is the documentation of Zingiberaceous plants used in Ayurveda, adding information to the systematics, vernacular names and chemistry with experimental data.Materials and Methods:The live plants were collected from wild and successfully conserved at Herbal Garden of Arya Vaidya Sala, Kottakkal. The experimental data of each species has been collected from the various sources. The photographs were taken and all relevant data documented.Results and Conclusion:A total of 13 species belonging to 7 genera of Zingiberaceae were documented. The work will be useful to students and researchers as it provides an easy access to Zingiberaceous plants used in Ayurveda.
A high-performance liquid chromatographic method for the estimation of berberine in the stem of Tinospora cordifolia (Willd.) Miers. ex Hook.f. and Thoms. and Tinospora sinensis (Lour.) Merrill is described. The dried stems of T. cordifolia and T. sinensis were defatted with petroleum ether (60-80°). The marc was dried and further extracted with methanol. The concentration of berberine in methanol extract was determined using a C-18 reverse phase column with a mobile phase of acetonitrile:water (10:90 v/v) at a flow rate of 0.6 ml/min and with UV detection at 266 nm. TLC and HPLC comparison of both the species revealed significant variation in the chemical constitution of the two species. This observation becomes important in the context of the use of T. sinensis in place of the genuine drug T. cordifolia.
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