HPLC estimation of berberine in <i> Tinospora cordifolia</i> and <i> Tinospora sinensis</i>

Abstract

A high-performance liquid chromatographic method for the estimation of berberine in the stem of Tinospora cordifolia (Willd.) Miers. ex Hook.f. and Thoms. and Tinospora sinensis (Lour.) Merrill is described. The dried stems of T. cordifolia and T. sinensis were defatted with petroleum ether (60-80°). The marc was dried and further extracted with methanol. The concentration of berberine in methanol extract was determined using a C-18 reverse phase column with a mobile phase of acetonitrile:water (10:90 v/v) at a flow rate of 0.6 ml/min and with UV detection at 266 nm. TLC and HPLC comparison of both the species revealed significant variation in the chemical constitution of the two species. This observation becomes important in the context of the use of T. sinensis in place of the genuine drug T. cordifolia.

Antifungal and high-performance liquid chromatography analysis of the crude extracts ofAcorus calamus, Tinospora cordifoliaandCelestrus paniculatus

“…HPLC was used for the estimation of berberine in the stem of T. cordifolia and Tinospora sinensis and the concentration of berberine was determined using a C-18 reverse phase column with a mobile phase of acetonitrile:water (10:90 v/v) at a flow rate of 0.6 ml/min and with UV detection at 266 nm. This observation becomes important in the context of the use of T. sinensis in place of the genuine drug T. cordifolia (Srinivasan et al 2008). A new sesquiterpene polyol ester characterised as 1a,6b,8b-triacetoxy-9b-benzoyloxydihydro-b-agarofuran, along with the three known compounds: 1a,6b,8a-triacetoxy-9a-benzoyloxydihydro-b-agarofuran, angulatueoid C and 1a,6b,8b,14-tetraacetoxy-9a-benzoyloxydihydro-b-agarofuran, was isolated from methanolic seed extract of C. paniculatus and these compounds showed a relaxant effect on the isolated rat ileum (Barbone et al 2007).…”

Validation of ethnomedicinal potential of Tinospora cordifolia for anticancer and immunomodulatory activities and quantification of bioactive molecules by HPTLC

“…Tinospora cordifolia (Amrita or Guduchi) is ethnomedicinally important and widely used plant in Ayurvedic system of medicine as general tonic, anticancer, antiulcer, antipyretic, immunomodulatory, hypoglycemic, anti-inflammatory, antiarithiritic, analgesic, diuretic and hepatoprotective potential (Srinivasan et al, 2008;Bhupindu et al, 1981;Rege et al, 1984). It has also been reported to be effective against throat cancer in humans (Chauhan, 1995).…”

“…Few reports are available for the quantification of some constituents in T. cordifolia using reverse phase high performance liquid chromatography (RP-HPLC) (Srinivasan et al, 2008;Alam et al, 2009a;Ahmad et al, 2006), liquid chromatography (RP-LC-DAD) with mass spectrometry (MS) (Patil et al, 2010), LC-ELSD method for simultaneous determination of ten sugars (Sharma et al, 2010a) and densitometric high performance thin layer chromatography (HPTLC) method (Puratchimani and Jha, 2007;Alam et al, 2009b]. In the last two decades, HPTLC is often used as an alternative to HPLC for the quantification of herbal products because of its simplicity, accuracy, and costeffectiveness, very less amounts of sample requirement, limited solvent waste and rapidity.…”

Evaluation of mammary gland immunity and therapeutic potential of Tinospora cordifolia against bovine subclinical mastitis

“…The herb is extensively used in the Indian System of Medicine; the extract of different parts of the herb has found wide use in variety of diseases. It is known for its immunomodulatory, antioxidant, and antibacterial properties (Srinivasan et al 2008).…”