Health service activities at the Clinical Laboratory produce infectious and non-infectious waste in the form of solid or liquid. Clinical laboratory liquid waste is one source of environmental pollution if the concentration exceeds the standard quality of wastewater. Polluted water quality parameters include microbiologics of Coli / Total Coliform Germs. Water quality with microbiological parameters can be used to determine the presence of bacteria, viruses, parasites. Bacteria used as indicators are Coliform bacteria. At present several clinical laboratories have implemented a Liquid Wastewater Treatment Plant. So that the waste discharged into the environment meets the standard quality standards for hospital liquid waste, and minimal pollution. This study aims to determine the quantity of Total Coliform at the inlet and outlet points of the Wastewater Treatment Plant Laboratory Medical Liquid. The results showed that the quantity of Total Coliform at the inlet sampling point of the liquid media waste at the Wastewater Treatment Plant Laboratory reached the highest value > 1.6 x 105 MPN / 100 ml, and after processing at the Wastewater Treatment Plant Laboratory decreased to 0 MPN / 100 ml (no coliform was found ), and with the highest value of 2.7 x 103 MPN / 100 ml, according to the waste water quality standard.
Pasir Kidul Village is one of the industrial villages in West Purwokerto District, Banyumas. Wood industrial waste (pencil factory), namely wood sawdust and wood chips accumulate and disturb the surrounding environment. One of the uses of this waste is the raw material for white mushroom baglog (Pleurotus ostreatus). This Community Partnership Program (PKM) aims to utilize wood sawdust for the cultivation of white oyster mushroom (Pleurotus ostreatus) in community groups in Pasir Kidul Village, West Purwokerto District, Banyumas Regency. This PKM is held for 5 months, from August to December 2018. There are 19 community groups in this community partnership program, with the target that after counseling, training and assistance in the application of "white oyster mushroom cultivation" technology can be applied to 80% of the program participants. The extension stages were training on how to make lemur and baglog for white oyster mushroom cultivation, training on planting seeds and maintaining white oyster mushroom cultivation media, counseling on the influence of types of growing media on white oyster mushroom production, and counseling on environmental factors as determinants of the success of white oyster mushroom cultivation. Some of the problems found by farmers who have carried out cultivation are Baglog sterilization and baglog rot. The results of the community partnership program show that there is an optimization of the utilization of wood sawdust waste which is abundant in the village, by processing wood sawdust into a growing medium for white oyster mushrooms (Pleurotus ostreatus) so that it has a higher useful value and selling value. Pasir Kidul adalah Kelurahan di Kecamatan Purwokerto Barat, Kabupaten Banyumas, Provinsi Jawa Tengah, Indonesia. Desa Pasir Kidul merupakan salah satu desa industri dengan terdapatnya industri pengolahan kayu (pabrik pensil) yang berdampak langsung dengan melimpahnya limbah padat berupa serbuk gergaji kayu dan serpihan kayu yang terbuang menumpuk di suatu lokasi tertentu yang dapat mengganggu kondisi lingkungan sekitar. Selama ini, serbuk gergaji kayu dibiarkan membusuk, ditumpuk dan dibakar yang berdampak negatif terhadap lingkungan sehingga penanggulangannya perlu dipikirkan. Salah satu upaya untuk membantu mengatasi permasalahan menumpuknya serbuk gergaji kayu di Desa Pasir Kidul adalah pemanfaatan serbuk gergaji kayu untuk budidaya jamur tiram putih (Pleurotus ostreatus) di kelompok masyarakat Desa Pasir Kidul Kecamatan Purwokerto Barat Kabupaten Banyumas. Upaya daur ulang serbuk gergaji kayu yaitu dengan memanfaatkan serbuk gergaji kayu tersebut untuk media tanam jamur tiram putih (Pleurotus ostreatus). Menurut Rachman dan Malik (2011), jika dilihat dari mata rantai industri pengolahan kayu, maka dalam industri penggergajian terjadi proses perubahan kayu pertama kali yaitu kayu dalam bentuk dolok menjadi kayu gergajian (sawn timber) atau disebut juga kayu konversi berupa papan, balok, tiang dan sortimen lainnya.
The city of Purwokerto is crossed by several large rivers, this research examines several tributaries of a large river. Some tributaries that pass through the city of Purwokerto are the Raden River, the Caban River, the Jurig River and the Luhur River. Tributaries have the potential to influence water quality. This study aimed to analyze the quantity of coliform bacteria as an indicator of water pollution in several tributaries in Purwokerto City, Banyumas Regency. Microbiological tests were carried out by calculating the total coliform quantity and fecal coliform using the Most Probable Number (MPN) test. Total coliform and fecal coliform can be used as a reference indicator of water quality in the presence of water pollution. The laboratory test results for the quantity of coliform bacteria showed in several tributaries in Purwokerto City, Banyumas Regency, very high in total coliform or fecal coliform, exceeding the water quality standard.
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