Quantity of Coliform Bacteria as Bioindicator of Water Pollution (Case Study: Several Tributaries in Purwokerto City, Banyumas Regency, Central Java)

Abstract

The city of Purwokerto is crossed by several large rivers, this research examines several tributaries of a large river. Some tributaries that pass through the city of Purwokerto are the Raden River, the Caban River, the Jurig River and the Luhur River. Tributaries have the potential to influence water quality. This study aimed to analyze the quantity of coliform bacteria as an indicator of water pollution in several tributaries in Purwokerto City, Banyumas Regency. Microbiological tests were carried out by calculating the total coliform quantity and fecal coliform using the Most Probable Number (MPN) test. Total coliform and fecal coliform can be used as a reference indicator of water quality in the presence of water pollution. The laboratory test results for the quantity of coliform bacteria showed in several tributaries in Purwokerto City, Banyumas Regency, very high in total coliform or fecal coliform, exceeding the water quality standard.

Analisis Most Probable Number (MPN) Coliform dan Escherichia coli Pada Air Sumur Bor di Pemukiman Warga Kelurahan Pucangsawit Surakarta

“…Faktor lain yang mempengaruhi tingginya nilai coliform di daerah pemakaman berhubungan dengan keberadaan bakteri hasil dari dekomposisi makhluk hidup yaitu manusia, hewan, dan tumbuhan 22 . Sisa-sisa makhluk hidup di pemakaman akan menimbulkan terjadinya kontaminasi air tanah oleh bakteri seperti misalnya Enterococcus spp, Escherichia spp termasuk Escherichia coli, dan KlebsiellaEnterobacter-Serratia (grup KES) 23 yang termasuk ke dalam fecal coliform 23,24 dan non-fecal coliform. Tabel 4 menunjukkan bahwa pada sampel air yang diambil dari sumur bor di sekitar pemakaman, nilai positif kontaminasi hanya ditemui pada kontaminasi coliform saja.…”