Water fluctuation and poor water management at swamp area caused rice plant is susceptible to flooding and submergence stress during plant growth and development. Rice plant which is submerged for long period caused plant wilt and die. Marker Assisted Backcrossing (MABc) is one of the rapid solution for introgression the submerged tolerant gene (sub-1) from donor parents to the local swamp rice varieties. It is expected that new swamp rice genotype will be able to adapt to stress submerged. The purpose of this researches were to select the heterozygous progenies from BC 2 F 1 generation based on backcrossing of 4 local parents of South Sumatera swamp rice genotypes i.e Siam, Pegagan, Pelita Rampak, and Payak Selimbuk with the donor of submergence tolerant gene (sub-1) of FR13A. Molecular analysis was studied by using foreground selection on sub-1 gene as a gene target or QTL, using 2 flanking markers based on microsatellite or Simple Sequence Repeat (SSR) of RM 23805 and RM 23915. The results of molecular analysis indicated that there were 37 number of progenies from total population of BC 2 F 1 generations. They were 8 numbers of BC 2 F 1 Siam from 24 progenies, 9 numbers of BC 2 F 1 Pegagan from 27 progenies, 10 numbers of BC 2 F 1 Pelita Rampak from 39 progenies, and 10 numbers of BC 2 F 1 Payak Selimbuk from 31 progenies which were identified as having sub-1 gene.
The development of a new submergence-tolerant variety is one ideal solution to reduce submergence stress impact caused by the unpredictable occurrence of flooding in the South Sumatra riparian wetland rice cultivation area. The Sub1 gene for submergence tolerance was introgressed into South Sumatra riparian wetland rice from the donor parent FR13A using marker-assisted backcrossing. This study involves a backcrossing between Pelita Rampak and BC1F1 Pelita Rampak, FR13A-derived Sub1 breeding lines. The main objective of this study was to introgress the Sub1 gene in BC2F1 using marker-assisted selection (MAS). The introgression of the Sub1 gene in the backcrossed lines was confirmed by the tightly linked markers RM219 and RM23915. The segregation ratio of RM219 was a good fit to the expected 1:1 Mendelian single-gene model (DF = 1.0, p ≤ 0.05). In the background study, out of 237 SSR markers unlinked to the target loci, 84 were found to be polymorphic between the two parents and were used for background selection among the selected progeny. Recurrent parent genome recovery in the backcrossed lines ranged from 57.1% to 72.6%. Improvements in the tiller number, percentage of filled grain, productive tiller number and percentage of tiller number were found on these backcrossed lines. The five best backcrossed lines were selected based on SSR markers, submergence tolerance, phenotypic study and agronomic performance.
Cekaman rendaman merupakan salah satu masalah utama yang dihadapi dalam kegiatan budidaya padi di lahan rawa lebak. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian fosfor sebelum tanaman terendam yang diharapkan mampu meningkatkan ketahanan tanaman padi terhadap cekaman rendaman. Penelitian disusun menggunakan Rancangan Petak Terbagi (Split Plot) dengan varietas padi sebagai petak utama dan waktu pemberian fosfor sebagai anak petak yang masing-masing diulang sebanyak 3 kali. Varietas padi yang digunakan yaitu IR 42, Inpari 30, dan Ciherang. Waktu pemberian fosfor terdiri dari kontrol (P0), pemberian fosfor 3 hari sebelum perendaman (P1), 7 hari sebelum perendaman (P2), dan 14 hari sebelum perendaman (P3). Hasil penelitian menunjukkan bahwa Ciherang merupakan varietas yang paling terhambat pertumbuhannya akibat cekaman rendaman. Selain itu, perlakuan P2 (aplikasi fosfor pada 7 hari sebelum perendaman) menunjukkan hasil terbaik dibandingkan perlakuan lain yang secara signifikan ditunjukkan pada peubah luas daun, berat kering akar dan total berat kering tanaman pada 6 minggu setelah tanam atau 14 hari setelah perendaman. Data yang dihasilkan diharapkan mampu menjadi bahan pertimbangan dalam perbaikan teknis budidaya padi di lahan rawa lebak. One of the major constraints of rice cultivation in the non-tidal swamp area is submergence stress, which is considered could be managed by phosphorus application before submergence in order to increase rice tolerance towards submergence stress condition. Split plot design was used in this research with three replicates. Rice cultivars served as the main plot consisted of IR42, Inpari 30, and Ciherang. While for the sub-plot was phosphorus application consisted of P0 = control, P1 = phosphorus application 3 days before submergence, P2 = phosphorus application 7 days before submergence, and P3 = phosphorus application 14 days before submergence. Results showed that Ciherang was the most affected cultivar due to submergence stress. Furthermore, P2had better performance compared to other P treatments as significantly showed in leaf area, dry root weight, and total dry weight at 6 weeks after sowing or 14 days after submergence. The resulted data hopefully can be used as a consideration for swamp rice cultivation improvement effort.
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