After separation of normal murine bone marrow cells in a Percoll density gradient cellular fractions with densities of 1.076 and 1.060 g/ml are capable of suppressing the in vitro growth of leukemia cells. The cytostatic activity of these fractions, however, does not surpass the level of antitumor antiproliferative activity intrinsic to intact bone marrow cells. These ceils were found to be capable of joining the splenocytes, thymoeytes, and lymph node cells in effeetor cytostatic cooperation and thus enhance the final antitumor effect.
The "graft versus leukemia" effect can be induced in (C57B1/6xDBA) F~ mice by two transplantations of cell suspensions containing bone marrow cells and splenocytes of (CBAxC57B1/6) FI mice and an injection of cyclophosphamide. The recipients of cell allotransplants were characterized by mixed chimeric hemopoiesis. These data outline a new approach to induction of the graft versus leukemia effect, requiring no complete elimination of the recipient's hemopoiesis and characterized by a low probability of lethal graft-versus-host reaction.
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