Bone marrow cells as cytostatic effectors responsible for suppressing leukemia growth in vitro

Селедцов, В. И.; Avdeev, Ilias V.; Селедцова, Г. В.; Самарин, Д. М.; Prokopenko, I. V.; Козлов, В. А.

doi:10.1016/0753-3322(96)82646-1

Cited by 11 publications

(16 citation statements)

References 15 publications

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“…and other soluble mediators. This is in line with our data [9] demonstrating the stimulating effect of recombinant human interleukin-2 on antitumor cytostatic activity of BMC.…”

Section: Resultssupporting

confidence: 94%

“…Together with our previous findings [9], these results suggest that T cells involved in immunogenesis increase (through the production of IFN-7, interleukin-2, and other mediators) the cytostatic activity of BMC and thus prevent the development of leukemia at the sites of intense hemopoiesJs. If so, the efficacy of BMC-mediated antitumor cytostatic mechanisms might increase during prolonged immune processes associated with the development or,/or example, chronic graft-versus-host reaction.…”

Section: Resultssupporting

confidence: 61%

“…T lymphocytes activated in an allogenic mixed culture produce mediator(s) stimulating the cytostatic activity of BMC, which is tested by suppression of P815 and L1310 cell growth in vitro [9]. Both T and B lymphocytes produce soluble factors potentiating the antitumor activity of BMC (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Tumor growth suppression by BMC is not associated with tumor cell destruction and is mediated, at least partially, by solutile cytostatic products [9].…”

Section: Abstract: T and B Lymphocytes; Bone Marrow Cell; Tumor Growmentioning

confidence: 99%

“…Bone marrow cells (BMC) phenotypically similar to bone marrow natural suppressor cells inhibit the growth of leukemic cells in vitro [1,2,[8][9][10]. Tumor growth suppression by BMC is not associated with tumor cell destruction and is mediated, at least partially, by solutile cytostatic products [9].…”

Section: Abstract: T and B Lymphocytes; Bone Marrow Cell; Tumor Growmentioning

confidence: 99%

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Activated lymphocytes produce mediators potentiating the antitumor cytostatic activity of bone marrow cells

et al. 1999

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Mitogen-activated murine T and B Iymphocytes produce soluble mediators that potentiate the inhibitory effect of bone marrow cells on the growth of leukemic cells in vitro. 7-Interferon is a T-cell product increasing the cytostatic activity of bone marrow cells. An increase in the cytostatic activity under the effect of T-cell soluble mediators was characteristic of bone marrow cells from nude mice. Key Words: T and B lymphocytes; bone marrow cell; tumor growth inhibitionBone marrow cells (BMC) phenotypically similar to bone marrow natural suppressor cells inhibit the growth of leukemic cells in vitro [1,2,[8][9][10]. Tumor growth suppression by BMC is not associated with tumor cell destruction and is mediated, at least partially, by solutile cytostatic products [9].We investigated the ability of activated T and B lymphocytes to regulate the cytostatic activity (CSA) of BMC by producing soluble factors. Cells were cultured in RPMI-1640 with 10 mM HEPES, 2 mM L-glutamine, 5x10 -5 M 2-mercaptoInstitute oi" Clinical Immunology, Siberian Division, Russian Academy of Medical Sciences, Novosibirsk ethanol, antibiotics, and 7% fetal calf serum (all reagents from Sigma) in a humidified atmosphere with 5% CO 2. MATERIALS AND METHODS(Splenocytes from normal BDF1 mice were activated with concanavalin A (Pharmacia, 5 ktg/ml) or lipopolysaccharide (E. coli 055 :B5:, Sigma, 20 ~tg/ml) in 25 cm2-plastic flasks (Linbro) (10 ml) tor 24 h. T and B lymphocytes activated with concanavalin A and lipopolysaccharide, respectively, were isolated by the positive penning method [3]. Immunofluorescence analysis of cells [3] showed that the purity of isolated T and B lymphocytes was close to 100%.For preparing culture supernatants, activated lymphocytes (3-4x106 cells/ml) were cultured in a 24-well plate (Linbro) for 24 h. The supernatant was obtained by centrifugation and stored at -20~ until use.In the cytostatic test, BMC (3xl05/well) were cultured with (25%) or without the supernatant from activated T or B lymphocytes in round-bottom 96-well plates (BDSL) tot 20 h. After the medium replacement, P815 or L1210 ceils were added to BMC into wells (104 cells/well) and cultured for 24 h. In the control tests, tumor cells were cultured without adding any cells or with thymocytes incapable of suppressing leukemic cell growth in vitro [1,8]. The level of proliferative activity was measured routinely by-incorporation of 3H-thymidin (Izotop) added in a dose of 0.75 lxCi to all wells 5 h belore the end of culturing.

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“…and other soluble mediators. This is in line with our data [9] demonstrating the stimulating effect of recombinant human interleukin-2 on antitumor cytostatic activity of BMC.…”

Section: Resultssupporting

confidence: 94%

Section: Resultssupporting

confidence: 61%

Section: Resultsmentioning

confidence: 99%

“…Tumor growth suppression by BMC is not associated with tumor cell destruction and is mediated, at least partially, by solutile cytostatic products [9].…”

Section: Abstract: T and B Lymphocytes; Bone Marrow Cell; Tumor Growmentioning

confidence: 99%

Section: Abstract: T and B Lymphocytes; Bone Marrow Cell; Tumor Growmentioning

confidence: 99%

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Activated lymphocytes produce mediators potentiating the antitumor cytostatic activity of bone marrow cells

et al. 1999

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Tumor Growth Inhibitory and Natural Suppressor Activities of Murine Bone Marrow Cells: A Comparative Study

Селедцов

Taraban

Селедцова

et al. 1997

Cellular Immunology

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Soluble factor and cell-cell interaction in cytostasis induced by bone marrow cells

et al. 2000

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Bone marrow cells as cytostatic effectors responsible for suppressing leukemia growth in vitro

Cited by 11 publications

References 15 publications

Activated lymphocytes produce mediators potentiating the antitumor cytostatic activity of bone marrow cells

Activated lymphocytes produce mediators potentiating the antitumor cytostatic activity of bone marrow cells

Tumor Growth Inhibitory and Natural Suppressor Activities of Murine Bone Marrow Cells: A Comparative Study

Soluble factor and cell-cell interaction in cytostasis induced by bone marrow cells

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