Rhizomania is one of serious threat to sugar beet production in Morocco and in several parts of the world. This disease led to a statistically significant decrease in the quality and yield of sugar beet plantations. Therefore, this study aimed at comparing the efficacy of six commonly used RNA extraction methods for the detection, recovery of RNA of beet necrotic yellow vein virus (BNYVV) and removal of amplification inhibitors by reverse transcription-polymerase chain reaction (RT-PCR). The efficiency of these extraction methods was then compared to that of a commercial isolation kit with high content of phenolic compounds. The results showed that the extraction with the lithium chloride technique, the commercial kit, and direct and membrane spotting crude extract methods were found effective in yielding a higher purity and a higher concentration of RNA when compared to the other tested methods. Extraction with the lithium chloride technique and the Qiagen kit (RNeasy Plant Mini Kit) allowed the most intense band, whereas the CTAB method has generated the least intense band. Furthermore, the silica capture extraction method did not yield any RNA after extraction and electrophoresis. Consequently, it was concluded that, of these six methods, the lithium chloride technique and the Qiagen kit are the most appropriate for the extraction of viral RNA from sugar beet samples prior to RT-PCR for detecting BNYVV.
IntroductionThe modernization of the arboriculture sector and its arboreal intensification requires the control of all production factors, including irrigation, fertilization and plant protection. The green Apple aphid, Aphispomi, is among the pests that must be watched and controlled.It can be developedin a fairly rapid way in favorable conditions. Thus, it is considered the second major scourge of Apple after Cydiapomnella. It is known by its polyphagia and mainly by its resistance to several active substances such as pyrethroid and neonicotinoid [1] . In addition to the Apple tree, on which the green apple aphid is considered very harmful, it also attacks other Rosaceae core and sometimes also other Rosaceaepip [2,3]. The stomata closure is the most important tool adopted by plants to reduce water losses in a situation of water stress [4] .This closure will result in physiological, morphological and phenological changes. The CO2 input is also prevented when the closure causes the disruption of the photosynthetic activity. Closing traps much of the energy to be dissipated by translation, which results in the increase of leaf temperature. Previous studies have shown the effect of aphids on plant photosynthesis [6] and on the induction of gene involved in the senescence of leaves [7]. In contrast, the abundance of aphids was affected by the physiological state of host plant [8], in particular, the nitrogen status in the plant [9,10]. Moreover, the water status of plant tissues can affect the growth of aphid by making it difficult of tap the phloem exudate [11]. In addition, several studies reported that development of pests is favored by the performance of plant growth [12,13] The soil and plants water stress influenced the growth rate of aphid's population on Cowpea. Thus, the number of aphids per plant was significantly lower in the aphid-resistant varieties than that observed in the aphidsusceptible varieties [4]. Therefore, the current study aimed at work we will evaluating the ability of strategically using nitrogen fertilization and irrigation practices through their positive impact on plant growth and water and nitrogen status of the plant tissues to control the green apple green aphid infestations. The impact of water and nitrogen depletion on AbstractThis work aims to establish an ecologically integrated strategy for pest management to assess the impact of water and nitrogen depletion on Aphis pomi infestation in the Apple orchard.The results of the study of the impact on the green Apple aphid show a significant effect on the availability of water and nitrogen on the level of Aphis pomi infestation. Indeed, the normal fertigation regime (T1 = 100%) proved to be more favorable to a prominent development of aphids, in comparison with a 25% water and nitrogen deficit regime (T2 = 75%), that minimize infestation. As for the evolution of wingless aphid and green aphid with wing, the Aphis pomi, at both regimes, the general shape of the evolution of winged curves looks like the one of the wingless ones, with...
Rhizomania is one of the most devastating diseases of sugar beet worldwide. The disease poses a serious threat to Moroccan production and it is capable of significantly decreasing quality and yield of sugar beet plantations. The long-term survival of its fungal vector (Polymyxa betae) in soil makes it a very difficult disease to manage. Therefore, this study investigated the potential of a non-pathogenic fungal Fusarium oxysporum strain Fo47 to control Polymyxa betae. This biocontrol agent was applied as soil treatment, seed treatment, or a combination of the both treatments. A bio-test was performed on treated soil. After four weeks of culture, the roots of sugar beet seedlings were retrieved and analyzed by the DAS-ELISA test. Results indicated that F. oxysporium Fo47 reduced the activity and survival of P. betae when compared to a reference biocontrol agent Trichoderma harzianum, which only revealed significant in reducing the viral load of Beet Necrotic Yellow Vein Virus (BNYVV) as seed treatment. The non-pathogenic Fusarium oxysporum Fo47 was more effective as soil treatment and allowed almost the same reduction of BNYVV virus concentration as T. harzianum 908. Therefore, our findings emphasizes that the performance of the biocontrol agent depends on the method of application.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.