Over the past decade, cold atmospheric plasmas have shown promising application in cancer therapy. The therapeutic use of plasma-activated media is a topic addressed in an emerging field known as plasma pharmacy. In oncology, plasma-activated media are  used to harness the therapeutic effects of oxidant species when they come in contact with cancer cells. Among several factors that contribute to the anticancer effect of plasma-activated liquid media (PALM), H2O2 and NO derivatives likely play a key role in the apoptotic pathway. Despite the significant amount of literature produced in recent years, a full understanding of the mechanisms by which PALM exert their activity against cancer cells is limited. In this paper, a sealed dielectric-barrier discharge was used to disentangle the effect of reactive nitrogen species (RNS) from that of reactive oxygen species (ROS) on cancer cells. Two cancers characterized by poor prognosis have been investigated: metastatic melanoma and pancreatic cancer. Both tumour models exposed to PALM rich in H2O2 showed a reduction in proliferation and an increase in calreticulin exposure and ATP release, suggesting the potential use of activated media as an inducer of immunogenic cell death via activation of the innate immune system.

Red squirrels (Sciurus vulgaris) are widely distributed throughout Eurasia, occurring in many types of coniferous and mixed-deciduous forests. In fragmented landscapes, small and partly isolated populations with low immigration rates show reduced genetic diversity, but reforestation can increase gene flow and restore levels of genetic variation in a few decades. No studies have so far investigated the genetic structure of red squirrel in large, continuous forests. The Italian Alps are presently characterized by almost continuous, recently reconnected forest habitats, that were affected by deep landscape changes during last glaciations but remained mostly unchanged between 10 000 and 200 years bp, when forest cover was heavily reduced. In this study we analyse patterns of genetic variability of red squirrels in and between seven sites distributed over 250 km of Alpine habitat, using mitochondrial DNA (mtDNA) and microsatellites. We use isolation-by-distance (IBD) models to investigate the relative importance that past (Pleistocene glaciations) and recent (fragmentation, bottlenecks) events had on the present genetic situation. Both nuclear and mtDNA data indicate a significant differentiation among study sites and a significant correlation between genetic and geographical distance only over a large scale. No recent bottlenecks are recorded through microsatellites and demographic models strongly support equilibrium between gene flow and drift; however, mtDNA suggests that there may have been local demographic crashes, probably in correspondence with the 19th-century forest fragmentation. These findings indicate that local landscape factors other than geographical distance per se, such as barriers of unsuitable habitat, affect gene flow and determine differentiation.

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This paper illustrates the results of an experimentation carried out by a multi-disciplinary research group made up of researchers from ITC-CNR of L'Aquila and of archaeologists of the University of L'Aquila. The research project carried out by the team is based on the analysis of the archaeological heritage (in particular, the documentation of some burials found in the medieval site of <i>Amiternum</i>, near L'Aquila). This starts from methods based on digital photogrammetric restitution, based on Structure from Motion (SfM) algorithms, and the generation of photorealistic textures in order to manage, in a 3D GIS environment, complex archaeological and anthropological data. The choice of technology to use is often determined by the specific needs of the survey, the purpose of the project, the budget and experience of the researchers, and the geometric characteristics of the assets, rather than the precision to be achieved. For the survey of the archaeological excavation of the medieval site of <i>Amiternum</i>, it was decided to use digital photogrammetry given that the objective was to document, with a rapid survey compatible with the times of an archaeological excavation the phases of image acquisition, processing and post processing of the site model. Furthermore, thanks to the integration of two technologies, digital photogrammetry and GIS, and the undisputed improvement in the management of 3D data by the GIS, three-dimensionality, in archeology in general, has become an indispensable component for site interpretation and for the documentation of the data.