In the recent years, the pollution of the environment by heavy metals has become a crucial problem across the world. Heavy metals consist of a group of metals and metalloids which have atomic density greater than 4000 kg m-3. Heavy metals such as Nickel (Ni), Cobalt (Co), Copper (Cu), Zinc (Zn), Lead (Pb) are present in the biota. These metals are also known as trace elements which play a very important role in various metabolic processes of plants, animals and microorganisms. Heavy metals may gain entrance into the human body through consumption of contaminated drinking water or ingestion of soil or crops grown on contaminated land. Heavy metals such as lead, mercury, cadmium and copper are collective poisons, which causes environmental hazards and are reported to be exceptionally toxic. These metals are important sources of oxidative stress in the cell and play an important role in a etiology of diverse human pathologies such as carcinogenesis. Exposure to heavy metal toxicity leads to brain damage, mental retardation, cerebral palsy, lung cancer, gastrointestinal abnormalities, dermatitis and death of the unborn fetus. Many metals have been shown to directly modify and/or damage DNA by forming DNA adducts that induce chromosomal breaks. The unrestricted access to the dumpsite means that each day, scavengers search for raw materials, much of which eventually finds its way back to neighborhoods as animal feed and even human food. Stray chicken, pigs, goats, dogs and cats roam the dumpsite eating the toxic matter and becoming vectors of pests and parasites that are eventually transferred to the surrounding home and hence causing diseases to both animals and human beings.
This investigation aimed at production of gluconic acid by fungal species isolated from soil in Keffi. Standard microbiological methods were employed for isolation and identification of the fungal isolates. The yields of gluconic acid produced by the different isolates of the fungi were determined using gas chromatograph and mass Spectrometry. The occurrence of fungi showed that Rhizopus oryzae was 100%, Aspergillus carneus was 75.0%, Aspergillus niger was 75.0% and Aspergillus terreus was 100% while Trichoderma viride was 25.0% and Fusarium moniliforme was 25.0%. The result further demonstrated that three species of the fungal isolates Aspergillus niger, Aspergillus carneus and Fusarium moniliforme were found to produce gluconic acid. Screening for gluconic acid production showed that Aspergillus carneus isolated from locations As1, Cs1 and Cs2, Aspergillus niger isolated from locations Bs1, Bs2 and Cs4 were able to produce gluconic acid. Result of effect of temperature, pH, substrate concentration and fermentation time on production of gluconic acid showed that Aspergillus niger Bs2 produced highest amount of gluconic acid at 28oC, similarly highest amount for gluconic acid produced by Aspergillus carneus As1 was at 28oC, whereas Fusarium moniliforme Bs4 produced highest at 30OC. pH 6.5 was found to the best optima pH for production of both gluconic acid for the fungi studied namely Aspergillus niger Bs2 and Aspergillus carneus As1 and Fusarium moniliforme Bs4 produced highest gluconic acid at pH 5.5. The substrate concentration showed highest production of gluconic acid was produced by Aspergillus niger Bs2 at substrate concentration of 25%. Aspergillus carneus As1 produced highest at substrate concentration of 20% and Fusarium moniliforme Bs4 produced highest at substrate concentration of 20%. The fermentation time showed highest production of gluconic acid by Aspergillus niger Bs2 and Aspergillus carneus As1 was after 144 hours whereas F. moniliforme Bs4 produced gluconic acid after 120hrs respectively. The fungi species isolated from soil in keffi revealed great ability in production of gluconic acid.
Over the years, there has been an increase in the rate of environmental pollution due to improper disposal and management of dumpsite waste. Leachate being generated as a result of the moisture associated within dumpsite, amount to contain various microbial pathogens and hazardous substance like cyanides, heavy metals and other chemicals acquired from the dumpsite. This can serve as a risk factor to the environment and public health when there is leachate migration. The migration of leachate into the environment potentially results in the contamination of soil, food products, animals, ground water, surface water and the air environment’ which in turn affect the health of human especially those who consumes from the contaminated products, those who inhabits around the dumpsite and other vulnerable like; young children, waste workers and immunocompromised persons. In Nigeria, though there are municipal waste dumpsites across the nation, these sites are below standard and are not properly managed. Being a great concern to the society, the need for proper design and reconstruction of a well-engineered landfill cannot be over emphasized, among others. This paper will therefore review the environmental and public health implication of dumpsite leachate so as to create more awareness on the need for proper waste management in Nigeria. It concludes by recommending some proper means of waste disposal and management which could be considered for a better health and environment in Nigeria.
Petroleum hydrocarbon is a major environmental pollutant throughout the world today because exploration and downstream utilization are associated with economic development. This work focus on effect of animal waste on bioremediation of soil contaminated spent hydrocarbon from auto mechanic workshop in Keffi. Isolation of bacteria and studies on utilized spent hydrocarbon supplemented with animal waste was carried out using standard microbiological methods and identified using 16s rRNA molecular techniques. The total spent hydrocarbon utilization was determined using Gas Chromatographic methods. The highest total bacteria count was recorded from High court (2.1 x105 cfu/g) and the lowest was from Keffi garage2 (1.2 x105 cfu/g). bacteria isolated and molecularly identified were Pectobacterium wasabiae, Pseudomonas fluorescens, and Priestia aryabhattai. The utilization of spent hydrocarbon after 4weeks of the experiment set up at ambient temperature Pseudomonas fluorescens recorded highest utilization of spent hydrocarbon amended with Poultry dropping and Cow dunk with 16.23±1.21 mg/ml and 13.44±0.23 mg/ml and Pectobacterium wasabia recorded the least with 8.12±0.20 mg/ml and 10.00±1.43. At temperature of 35 ℃ Pseudomonas fluorescens recorded highest utilization of spent hydrocarbon amended with Cow dunk and Poultry dropping with 17.13±1.11 mg/ml and 15.14±1.13 mg/ml while Priestia aryabhattai recorded the lowest from Poultry dropping with 6.10±0.06 mg/ml and Cow dunk with 8.23±0.03 mg/ml. at 40 ℃ the highest utilization was recorded by Priestia aryabhattai from Cow dunk amended spent hydrocarbon with 18.13±1.01 mg/ml and Poultry dropping with 16.24±1.33 mg/ml. from the findings of this study temperature plays a key role spent hydrocarbon that containment our environmental
To counter the effects of climate change due to use of fossil fuels, much attention has been placed into the production of biomass-derived fuels. This investigation was aimed at producing biodiesel from microalgae species isolated from water samples in Keffi, Nasarawa state. Standard microbiological methods were used for isolation and identification of the microalgal isolates. The percentage occurrence of the isolates showed that Chlorella vulgaris had a 100% occurrence with Chlorella lewinii and Stigeoclonium tenue having a 50% occurrence each while Cladophora glomerata having a 25% occurrence. The growth curve showed that the micro algal isolates experienced exponential growth under daily sunlight exposure. The effect of temperature on the biomass and lipid extracted showed that Chlorella vulgaris, Chlorella lewinii and Stigeoclonium tenue had the highest biomass yields of 8.01g/l, 12.17 g/l and 15.10 g/l respectively and lipid production of 3.30 µg/l, 3.11 µg/l and 5.11 µg/l respectively at 32 ℃. Further increase in temperature led to decrease in their biomass yields and lipid production. The effects of pH showed that all the microalgal isolates had its highest biomass yields and lipid production in an alkaline pH of 7.5 but when the pH was reduced up to 4.5, the resultant microalgal biomass yields and lipid production of the microalgal isolates decreased. The fatty acid profiles of the biodiesel derived from the microalgal isolates showed that it was composed mainly of palmitic, oleic, Myristic and Stearic acids which made it a viable fuel source.
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