Effect of animal waste on bioremediation of soil contaminated with spent hydrocarbon from auto mechanic workshop in Keffi, Nasarawa State, Nigeria

Abstract

Petroleum hydrocarbon is a major environmental pollutant throughout the world today because exploration and downstream utilization are associated with economic development. This work focus on effect of animal waste on bioremediation of soil contaminated spent hydrocarbon from auto mechanic workshop in Keffi. Isolation of bacteria and studies on utilized spent hydrocarbon supplemented with animal waste was carried out using standard microbiological methods and identified using 16s rRNA molecular techniques. The total spent hydrocarbon utilization was determined using Gas Chromatographic methods. The highest total bacteria count was recorded from High court (2.1 x105 cfu/g) and the lowest was from Keffi garage2 (1.2 x105 cfu/g). bacteria isolated and molecularly identified were Pectobacterium wasabiae, Pseudomonas fluorescens, and Priestia aryabhattai. The utilization of spent hydrocarbon after 4weeks of the experiment set up at ambient temperature Pseudomonas fluorescens recorded highest utilization of spent hydrocarbon amended with Poultry dropping and Cow dunk with 16.23±1.21 mg/ml and 13.44±0.23 mg/ml and Pectobacterium wasabia recorded the least with 8.12±0.20 mg/ml and 10.00±1.43. At temperature of 35 ℃ Pseudomonas fluorescens recorded highest utilization of spent hydrocarbon amended with Cow dunk and Poultry dropping with 17.13±1.11 mg/ml and 15.14±1.13 mg/ml while Priestia aryabhattai recorded the lowest from Poultry dropping with 6.10±0.06 mg/ml and Cow dunk with 8.23±0.03 mg/ml. at 40 ℃ the highest utilization was recorded by Priestia aryabhattai from Cow dunk amended spent hydrocarbon with 18.13±1.01 mg/ml and Poultry dropping with 16.24±1.33 mg/ml. from the findings of this study temperature plays a key role spent hydrocarbon that containment our environmental