Binara (Artemisia vulgaris L.) merupakan salah satu jenis tumbuhan yang dimanfaatkan sebagai obat dan banyak terdapat di Kabanjahe, Kabupaten Karo, Sumatera Utara. Masyarakat Karo secara turun temurun menggunakan tanaman ini sebagai obat diare.Penelitian ini bertujuan untuk mengetahui daya hambat antibakteri dan aktivitas antioksidan dari ekstrak daun binara hasil blanching yang menggunakan pelarut air dalam proses ekstraksi secara refluks. Uji aktivitas antibakteri dilakukan terhadap bakteri Escherichia coli ATCC 25922 (gram negatif) menggunakan metode difusi. Ekstrak air daun binara yang diuji adalah konsentrasi 0%; 2,5%; 5%. Aktivitas antibakteri diukur dari luas diameter zona bening disekitar sumur. Hasil penelitian menunjukkan ekstrak air daun binara memiliki aktivitas antibakteri berturut-turut pada konsentrasi 0%; 2,5%; dan 5% adalah 0 mm; 18 mm; dan 25,2 mm. Sebagai kontrol positif digunakan kloramfenikol dan diperoleh daya hambat antibakterinya sebesar 28 mm. Uji antioksidan dilakukan dengan metode peredaman DPPH (1,1-diphenyl-2-picrylhydrazil). Hasil penelitian menunjukkan aktivitas antioksidan ekstrak air daun binara termasuk sedang (% inhibisi 45,24% dan IC50 114,23 ppm) dan sebagai kontrol positif digunakan vitamin C (% inhibisi 56,27% dan IC50 72,75 ppm). (% inhibition 45.24% and IC50 114.23 ppm) and as positive control used vitamin C (% inhibition and 56.27% and IC50 72.75 ppm).
Kata kunci: antibakteri, antioksidan, Artemisia vulgaris L., Escherichia coli ATCC 25922, metode DPPH
Binara (Artemisia vulgaris L.) is one of the types of plant sused as medicine in
The Crinum plant varieties found, called "Ompu-ompu", identified as a type of Crinum asiaticum-L which used as a drug. The antioxidant, toxicity and antibacterial of the Crinum leaves and bulbs extracted with ethanol and treated by DPPH (2-2 diphenyl 1-1-picrylhydrazyl) method, Brine Shrimp Lethality Test (BSLT) method, and diffusion method respectively. It was found that both the leaves and bulbs have an antioxidant site with 54.21 ppm and 33.79 ppm respectively through IC50 test 25.8 ppm which used vit.C as a control. Furthermore, it was found that their toxicity measured by LD50 is 243.331 and 507.838 respectively. In addition, the antibacterial test showed that the widest inhibition of the leaves and bulbs are 15.7 mm and 12.4 mm against Escherichia coli respectively and 12.3 mm and 11.5 mm against Staphylococcus aureus. Phytochemical screening indicated the presence of alkaloids, flavonoid, saponins and tannins.
Inhibitory compound of α-glucosidase bioactivity was isolated from ethanol extract of the stem bark of raru (Vatica pauciflora Blume) which, is a wild plant that grows in Tapanuli state in Indonesia.The ethanol extract of the stem bark of raru was partitioned and was chromatographed on columns with stationare fase silica gel 60 mesh F 254 (0.063 mm-0.200 mm). The ethanol extract of isolates showed the enzyme α-glucosidase inhibitor IC 50 of 93.46. The next step is the determination of the chemical structure of the Ultraviolet-visible spectroscopy (UV Vis); FT-IR (Fourier Transform Infrared spectroscopy), NMR (Nuclear Magnetic Resonance) namely COSY, Heteronuclear Multiple-Quantum Correlation (HMQC); and Heteronuclear Multiple-Bond Correlation (HMBC).
This research is a research on the development of Website learning media. This study aims to produce a product in the form of website learning media on thermochemical material that has been developed and its effect on student learning outcomes. This research was conducted by analyzing several Thermochemical learning websites with good ratings by 15 teachers who teach Chemistry in Serdang Bedagai Regency. From this analysis, it was obtained that the average count for Website A was 3.64 and Website B was 3.83, with sufficiently valid information but still requiring improvement in its presentation. The results of the analysis become a reference point in developing the Website learning media. The website that has been developed is analyzed according to the BSNP standard by 2 IT learning experts with an average result of 4.76 with very valid criteria. In addition, 15 teachers obtained an average score of 4.74 with very valid criteria. Limited trials of student learning outcomes were analyzed using independent t-test, with a value of t-count> t-table (3.89> 1.70), which means that with the implementation of integrated website media the inquiry learning strategy is higher than the KKM of the school at the level of significance of 0.05.
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