Glucosinolates are anti-nutritional factors present abundantly in the seed meal fraction of oilseed Brassica species. They are found in varying levels among different genotypes. Those genotypes containing less than 30 µmol/ g are considered low/zero glucosinolate type and are preferred for edible purposes due to low pungency. Twenty two different genotypes were taken for the analysis of glucosinolates by spectrophotometry. A regression model was obtained using Ordinary Least Square technique which predicted a formula. Total glucosinolates (µmol/g) = 1.40 + 118.86 × A 425 , where A 425 is the absorbance at 425 nm. The total glucosinolate content obtained by the prediction formula when compared with HPLC data showed a correlation coefficient of 0.942. This high correlation between the two data sets validated the developed methodology. This method also simplifies the estimation of total glucosinolates by excluding the use of HPLC or other sophisticated instruments.
A water-deficit stress (WDS)-responsive transcription factor-encoding gene isolated from drought-tolerant Oryza sativa L. cv. N-22 corresponding to the AP2/ERF family and named AP2/ERF-N22, when overexpressed in Arabidopsis under a constitutive promoter, showed improved turgor and less wilting as compared to wild-type plants under WDS. However, the transgenic plants showed phenotypic aberrations such as stunted growth, smaller silique size, reduced number of seeds and delayed flowering. Physio-biochemical analysis of T2 plants revealed higher relative water content, membrane stability index, capacity to scavenge reactive oxygen species and increased levels of proline, carotenoids, wax and abscisic acid content under WDS. Higher abscisic acid content resulted in reduced stomatal conductance and transpiration rate. The reduced chlorophyll content also leads to reduced photosynthetic rate and efficiency. Thus, this gene may be exploited for enhanced drought tolerance in crop plants.
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