Isolation and characterization of an AP2/ERF-type drought stress inducible transcription factor encoding gene from rice

Mawlong, Ibandalin; Ali, Kishwar; Kurup, Devika B.; Yadav, Sangita; Tyagi, Aruna

doi:10.1007/s13562-012-0185-3

Cited by 18 publications

(18 citation statements)

References 33 publications

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“…AP2/ERF-N22, having ABRE and DRE cis elements within 500 bp upstream of the initiation codon, has previously been identified and characterized (Mawlong et al 2014). The annotated gene from Oryza sativa ssp.…”

Section: Discussionmentioning

confidence: 99%

“…The AP2/ERF-N22 sequence was submitted to GenBank (accession #EF362638). Northern and RT-PCR analysis showed that transcript of AP2/ERF-N22 accumulates in response to drought stress, and Southern blot analysis indicated the presence of a single copy of AP2/ERF-N22 in the O. sativa genome (Mawlong et al 2014). Nakano et al (2006) performed a computational analysis of the complete AP2/ERF superfamily in Arabidopsis and rice and divided it into fourteen groups.…”

Section: Discussionmentioning

confidence: 99%

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Functional validation of a drought-responsive AP2/ERF family transcription factor-encoding gene from rice in Arabidopsis

et al. 2015

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A water-deficit stress (WDS)-responsive transcription factor-encoding gene isolated from drought-tolerant Oryza sativa L. cv. N-22 corresponding to the AP2/ERF family and named AP2/ERF-N22, when overexpressed in Arabidopsis under a constitutive promoter, showed improved turgor and less wilting as compared to wild-type plants under WDS. However, the transgenic plants showed phenotypic aberrations such as stunted growth, smaller silique size, reduced number of seeds and delayed flowering. Physio-biochemical analysis of T2 plants revealed higher relative water content, membrane stability index, capacity to scavenge reactive oxygen species and increased levels of proline, carotenoids, wax and abscisic acid content under WDS. Higher abscisic acid content resulted in reduced stomatal conductance and transpiration rate. The reduced chlorophyll content also leads to reduced photosynthetic rate and efficiency. Thus, this gene may be exploited for enhanced drought tolerance in crop plants.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Functional validation of a drought-responsive AP2/ERF family transcription factor-encoding gene from rice in Arabidopsis

et al. 2015

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“…Tomato SlSHINE3 is involved in cutin metabolism of fleshy fruit epidermal cells for patterning the epidermal surface (Shi et al, 2013) and SlSHN1 is involved in wax accumulation of leaf epidermal cells, which enhances drought tolerance (Al-Abdallat et al, 2014). Rice OsAP2/ERF-"N-22" is involved in wax biosynthesis and also enhances drought resistance (Mawlong et al, 2014), while wheat TdSHN1 is involved in the cuticle formation of leaf surfaces (Jäger et al, 2015). Eucalyptus EgrSHN1 and EgrSHN2 are involved in cell wall biosynthesis of flowers (Marques et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

PeERF1, a SHINE-Like Transcription Factor, Is Involved in Nanoridge Development on Lip Epidermis of Phalaenopsis Flowers

et al. 2020

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Phalaenopsis orchids have a spectacular floral morphology with a highly evolved lip that offers a landing platform for pollinators. The typical morphological orchid lip features are essential for the special pollination mechanism of Phalaenopsis flowers. Previously, we found that in the lip, a member of the AP2/EREBP protein family was highly expressed. Here, we further confirmed its high expression and characterized its function during lip development. Phylogenetic analysis showed that AP2/EREBP belongs to the Va2 subgroup of ERF transcription factors. We named it PeERF1. We found that PeERF1 was only expressed at stage 5, as flowers opened. This coincided with both thickening of the cuticle and development of nanoridges. We performed knockdown expression of PeERF1 using CymMV-based virus-induced gene silencing in either the AP2 conserved domain, producing PeERF1_AP2-silenced plants, or the SHN specific domain, producing PeERF1_SHN-silenced plants. Using cryo-SEM, we found that the number of nanoridges was reduced only in the PeERF1_AP2-silenced group. This change was found on both the abaxial and adaxial surfaces of the central lip lobe. Expression of PeERF1 was reduced significantly in PeERF1_AP2-silenced plants. In cutin biosynthesis genes, expression of both PeCYP86A2 and PeDCR was significantly decreased in both groups. The expression of PeCYP77A4 was reduced significantly only in the PeERF1_AP2-silenced plants.Although PeGPAT expression was reduced in both silenced plants, but to a lesser degree. The expression of PeERF1 was significantly reduced in the petal-like lip of a big-lip variant. PeCYP77A4 and PeGPAT in the lip were also reduced, but PeDCR was not. Furthermore, heterologous overexpression of PeERF1 in the genus Arabidopsis produced leaves that were shiny on the adaxial surface. Taken together, our results show that in Phalaenopsis orchids PeERF1 plays an important role in formation of nanoridges during lip epidermis development.

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“…Many of the stress-inducible AP2, ERF and DREB subfamily members have been isolated and characterized. They can modulate transcription of downstream genes involved in drought tolerance ( Mawlong et al, 2014 ). Regulation of transcription is a common target to modulate gene expression both under abiotic and biotic stresses.…”

Section: Introductionmentioning

confidence: 99%

Elicitor-Induced Biochemical and Molecular Manifestations to Improve Drought Tolerance in Rice (Oryza sativa L.) through Seed-Priming

et al. 2017

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Rice (Oryza sativa L.) is one of the major grain cereals of the Indian subcontinent which face water-deficit stress for their cultivation. Seed-priming has been reported to be a useful approach to complement stress responses in plants. In the present study, seed-priming with hormonal or chemical elicitor [viz. methyl jasmonate (MJ), salicylic acid (SA), paclobutrazol (PB)] showed significant increase in total phenolic content, antioxidant activity and expression of Rice Drought-responsive (RD1 and RD2) genes (of AP2/ERF family) in contrasting rice genotypes (Nagina-22, drought-tolerant and Pusa Sugandh-5, drought-sensitive) under drought stress. However, decrease in lipid peroxidation and protein oxidation was observed not only under the stress but also under control condition in the plants raised from primed seeds. Expression analyses of RD1 and RD2 genes showed upregulated expression in the plants raised from primed seeds under drought stress. Moreover, the RD2 gene and the drought-sensitive genotype showed better response than that of the RD1 gene and the drought-tolerant genotype in combating the effects of drought stress. Among the elicitors, MJ was found to be the most effective for seed-priming, followed by PB and SA. Growth and development of the plants raised from primed seeds were found to be better under control and drought stress conditions compared to that of the plants raised from unprimed seeds under the stress. The present study suggests that seed-priming could be one of the useful approaches to be explored toward the development of simple, cost-effective and farmer-friendly technology to enhance rice yield in rainfed areas.

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Isolation and characterization of an AP2/ERF-type drought stress inducible transcription factor encoding gene from rice

Cited by 18 publications

References 33 publications

Functional validation of a drought-responsive AP2/ERF family transcription factor-encoding gene from rice in Arabidopsis

Functional validation of a drought-responsive AP2/ERF family transcription factor-encoding gene from rice in Arabidopsis

PeERF1, a SHINE-Like Transcription Factor, Is Involved in Nanoridge Development on Lip Epidermis of Phalaenopsis Flowers

Elicitor-Induced Biochemical and Molecular Manifestations to Improve Drought Tolerance in Rice (Oryza sativa L.) through Seed-Priming

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