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KeyWorcls Column liquid chromatography Dansylation BiogenTc amTnes Wines and Beers SummaryA sensitive hTgh performance liquid chromatographic method for the sTmuhaneous determTnation of eleven biogenic amines, using 1,7-diaminoheptane as internal standard, has been developed. The method involves pre-column derTvatizatTon of the amTnes with dansyl chlorTde and subsequent solid phase extraction of the derivatives through C18 cartridges. The derivati zation and solid phase extraction procedures were optimized. The separation of dansyla mTdes was achieved on an InertsTI ODS-3 column (250 x 4 mm I.D., 5 am) using a 35-rain gradient elution method wth a binary system of aoetonitrile-water, a flow rate of 1 mL.min 1 with UV detection at 254 nm. Linearity of derivatTzation was obtaTned for concentrations rangTng from 0.025 to 3.0 rag./ 1. The within and belween day relative standard deviations ranged from 0.4 to 5.7% and 0.6 to Z3% respectively The overall process was successfully applied to identi[y and quanti~ bTogenTcamines Tn white, red and Retsina Greekwnes and Greek beers, a fier theTr treatment with polyvinylpyrrolido ne.

The frequency and severity of crop protection product (pesticide) contamination of peaches grown conventionally were compared with those of peaches grown by integrated crop management (ICM). The peach samples (n = 150) were collected preharvest (June-August 2001) from both conventional (n = 55) and ICM (n = 95) cultivations from the Pella and Imathia districts of Macedonia, Northern Greece. The residue levels of selected insecticides, fungicides and acaricides in peach samples were determined by gas chromatography-mass spectrometry following solid-phase extraction. The concentrations of all detected pesticides were lower than the maximum residue limits (MRLs) in all peach samples grown with the ICM system (p<0.001). However, chlorpyrifos residues at levels higher than the MRLs were detected in four peach samples (i.e. 7% of the total samples) grown by the conventional system. Comparing the results for both cultivation methods with the reported average percentage (3.6%) of fruit samples with pesticide residues above the MRLs (European Union report for Greece in 2001), it was concluded that the initial implementation of the ICM in Greece was successful. The present study indicates that ICM cultivation has a higher efficiency in terms of product safety and quality. Furthermore, the results suggest that the application of conventional cultivation requires continuous monitoring of various crop protection product levels.