Tripeptides T-36 and, particularly, T-38 in concentrations of 0.1, 1, and 10 ng/ml inhibited proliferation of primary trypsinized embryonic mesenchymal stem cells, rat transplantable KF-1 fibroblasts, and human erythromyelosis K-562 cells. Inhibition of proliferation in embryonic and immortalized cells under the influence of tripeptides probably reflects antitumor activity of these substances. Tripeptides had no effect on lymphocyte survival and their adhesive, cytotoxic, and induced proliferative activities. T-36 did not modulate the proliferative properties of erythromyelosis K-562 cells. Tripeptides did not change engulfment activity and spontaneous and induced bactericidal activities of granulocytes. T-36 in a concentration of 0.1 ng/ml increased spontaneous proliferation of normal lymphocytes. These data suggest that tripeptides stimulate nontumor immune cells in adult people.
Purpose of the study. The sepsis differentiation
criteria and metabolically induced diabetic foot
lesions must be established.
Material and methods. The 115 patients were
observed.
Results and discussion. Sepsis by the qSOFA
criteria was diagnosed in 3, heart failure of 3–4 class
by NYHA classification estimated in 39 cases. High
limb amputation had been performed to 18 patients,
surgery on the foot to 97. Foot lesion relapse
accompanied by unstable glycaemia had been
observed in 25 cases. For the surgical correction of
the diabetes ileoduodenoplasty had been performed
in 7 cases, which resulted in uncomplicated wound
healing in all patients.
Conclusion. Sepsis progression in patient with
diabetic foot case proof indicates the necessity high
lower limb amputation. Foot lesion relapse after the
effective surgical sanitation indicate on metabolic
disorder, not on sepsis. Surgical correction of the
diabetes by mean of ileoduodenoplasty performing
seems to be the reliable method of foot lesion relapse
preventing.
Keywords: diabetic foot, sepsis, metabolism
challenge, surgical correction, ileoduodenoplasty.
To investigate the effect of pre-transplantation of multipotent stromal cells (MSCs) of bone marrow on gastric ulcer formation and the state of
the immune system in conditions of acute and prolonged stress. Wistar rats reproduced immobilizing water-immersion stress of 2 types: acute and prolonged. Investigated the number and area of stress ulcers, thymus and spleen, as well as hematologic parameters, proliferative and cytotoxic activity of peripheral blood mononuclear cells, splenocytes and cells of lymph nodes, determined the absorption activity of neutrophils. With prolonged stress as a result of MSC transplantation, the number and area of ulcers significantly decreased, indicating the adaptive protective effect of cells. With acute stress, the introduction of MSC had virtually no effect on ulcer formation. With prolonged stress, there was a decrease in thymus, spleen and leukocyte counts in the blood. Under the influence of transplanted MSCs, the number of all mobilized cells was normalized with the exception of lymphocytes. The natural cytotoxicity and proliferative activity of splenocytes, cells of lymph nodes and peripheral blood in acute and prolonged stress as a result of the introduction of MSC did not change significantly. The introduction of bone marrow MSС 24 h before the last reproduction of stress responses in the model of prolonged stress significantly reduced the number and area of ulcers, which generally indicates the anti-ulcer effect of cells, and normalized the stress-induced quantitative cellular changes in the immune system. Transplantation of bone marrow MSCs to rats prior to reproduction of stress enhances the adaptive antistress mechanisms that develop during prolonged stress, leading to suppression of gastric ulcer formation and significantly altering immune system activity. It can be assumed that one of the mechanisms of action on the body of MSCs is to promote the formation of adaptive responses.
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