1. The membrane structure of erythrocytes of rats with different forms of arterial hypertension was studied by means of two hydrophobic fluorescent probes (diphenylhexatriene and pyrene). 2. Microviscosity of hydrophobic areas of erythrocyte membrane of spontaneously hypertensive rats was found to be increased compared with that of membranes from normotensive control rats. 3. No alterations of membrane structure of erythrocytes of deoxycorticosterone-treated rats and renal hypertensive rats were found.
Using flow cytometry and fluorescence polarization analysis, specific muramyl peptide‐binding sites were shown to be located inside T‐lymphocytes, macrophages and neuroblastoma cells, but not inside B‐cells. No binding sites were found on the cell surface. The number of binding sites for each cell type was determined. Two types of binding sites were observed for myelomonocytic WEH1‐3 cells with K
fd values of 21 and 540 nM. Inhibition analysis demonstrated that for effective binding, an intact glycopeptide molecule and D‐configuration of isoglutamine residue are important.
The comparative analysis of responses of memory and naive T lymphocytes to Ca P+ -mobilizing agents, namely Con A, thimerosal, thapsigargin and ionomycin, was carried out. The effect of these agents on both types of T cells differed qualitatively and quantitatively. The lack of intracellular Ca P+ stores in memory T cells was shown. Ca P+ -mobilizing agents did not induce influx of Ca P+ in memory T cells from outside and this was the reason for their stability to Ca P+ ionophores. It was also shown that memory T cells were resistant to the`Ca P+ paradox'.z 1999 Federation of European Biochemical Societies.
Immunological memory was reproduced and studied by subcutaneous vaccination of mice with attenuated strain of Mycobacterium bovis. Ionomycin-resistant cells of CBA mice were for the first time isolated from immune cells and studied as memory T-cells. Previously they were described for another experimental model. They are characterized by high expression of CD3, CD4, CD8, CD28, and ~/13-T-cell receptor. The capacity of splenocytes and ionomycin-resistant cells to adoptive transfer of antituberculosis resistance to intact recipients is studied.
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