Immunological memory induced byMycobacterium bovis in inbred mice

Abstract

Immunological memory was reproduced and studied by subcutaneous vaccination of mice with attenuated strain of Mycobacterium bovis. Ionomycin-resistant cells of CBA mice were for the first time isolated from immune cells and studied as memory T-cells. Previously they were described for another experimental model. They are characterized by high expression of CD3, CD4, CD8, CD28, and ~/13-T-cell receptor. The capacity of splenocytes and ionomycin-resistant cells to adoptive transfer of antituberculosis resistance to intact recipients is studied.

A comparative study of the calcium system in memory T cells and naive T cells

“…While we cannot entirely rule out the idea that the brief exposure to ionomycin might induce some long‐lasting change in cell behavior that alters subsequent responsiveness (up or down), and that the variations in responsiveness we see might not reflect intrinsic differences between naive and memory T cells themselves, we have several reasons for discounting this. Firstly, the ionomycin‐exposed T cells respond strongly to specific antigens under culture conditions [3, 4, 16, 23]. The ionomycin‐resistant T cells are able to confer resistance to Neisseria meningitidis [5]and Mycobacterium tuberculosis infections in mice [4].…”

“…Firstly, the ionomycin‐exposed T cells respond strongly to specific antigens under culture conditions [3, 4, 16, 23]. The ionomycin‐resistant T cells are able to confer resistance to Neisseria meningitidis [5]and Mycobacterium tuberculosis infections in mice [4]. Furthermore, the CD44 low naive T cells are more sensitive to ionomycin [25], and might therefore be supposed to be more likely to be damaged by exposure to this agent, and yet are found to contain most Con A‐responsive cells.…”

“…It has also been shown by using limiting dilution analysis that the Ag‐specific helper memory T cells were found predominantly in the ionomycin‐resistant fraction of the spleen T lymphocytes [3]. Recently, using adoptive cell transfer and following infection challenge, we have shown that ionomycin‐resistant T cells can transfer the resistance to Neisseria meningitidis and Mycobacterium tuberculosis infections [4, 5]. Taking together these experiments have led to the suggestion that these ‘ionomycin'resistant’ T cells might correspond to memory T cells.…”