The purpose of this study was to determine the effect of the partial or total replacement of the inorganic Cu and Fe salts by organic forms of these elements on broiler performance and on the elimination of minerals through broiler droppings. A study was conducted for 42 days on 240 broiler chicks treated with trace elements chelates with amino acids: B-TRAXIMRTEC Cu-I30; B-TRAXIMRTEC Fe-120. The broiler chicks were housed in cages (10 chicks per cage, 6 cages per group) and assigned to 4 groups (C, E1, E3 and E3) fed on the same corn-soybean meal-based diet. Phased-feeding was used according to the developmental stages of the broilers (starter, grower and finisher). Feed intake and the amount of droppings were recorded daily. The daily records of droppings and their chemical analysis was used to determine the trace elements load of the droppings. The total or partial replacement of the inorganic Cu and Fe salts by chelates of these minerals with amino acids in broiler diets didn't affect broiler performance. The inclusion of Cu chelates in broiler diets at the level recommended by the manufacturer of by NRC decreased by at least 34% the level of Cu (environmentally toxic element) in the droppings. The corresponding decrease was of 5-21% for Fe.

Protein quality is determined by the type and concentration of the essential amino acids and by their bioavailability. Hence, the content of dietary essential amino acids from a protein or mixture of proteins is a factor which determines the feeding quality of the protein. The purpose of the study was to evaluate the quality of the protein from a high protein raw ingredient (corn gluten) using a chromatographic method (HPLC) under optimized experimental conditions. In this study, we used pre-column derivatization, separation by high-performance liquid chromatography (HPLC) and UV detection. The optimized method was used to determine the amino acids from high-protein raw ingredients commonly used in animal feeding (corn gluten, 61%CP). A set of 6 amino acids analyses has been performed in 6 different days, each sample being prepared in double. For characterization of data strings we used quality parameters: average, standard deviation, standard error, confidence level, precision, accuracy. For verifying the Gaussian shape of the strings we used the Kernel Density. For identifying and rejecting the outliers from the data strings we used the Q test. The very low values of the accuracy for several amino acids (glutamic acid, threonine, alanine, valine, phenylalanine, isoleucine, leucine) determined us to introduce the dilution stage (1:2) for the hydrolysed samples. The method can be considered as repeatable (precision in different days) and accurate (evaluation by tracing yields) for all the determined amino acids.