OBJECTIVE: The purpose of the present study was to investigate the presence of insulin‐like growth factor‐I (IGF‐I) in the labial salivary glands of patients with Sjögren's syndrome and healthy controls and to determine if there are any differences between these two groups.
DESIGN: An immunohistochemical study.
SUBJECTS AND METHODS: Twenty‐five patients with Sjögren's syndrome, 20 healthy controls and 20 patients with mucoceles of the lip were used in this study. All individuals underwent a systemic evaluation and a lip biopsy. Sections from the lip biopsies were stained with haema‐toxylin and eosin (H&E). Immunohistochemical staining was also performed using a three‐step indirect immunoperoxidase for IGF‐I.
RESULTS: The light microscopic examination revealed the presence of a mononuclear infiltration in the labial salivary glands of patients with Sjögren's syndrome. Most of the infiltrates were lymphocytes. Immunohistochemically an intense staining result was apparent in the same group. In contrast sections of labial salivary glands of healthy individuals and of patients with mucoceles revealed very weak staining.
CONCLUSIONS: The above findings and the fact that both lymphocytic infiltration and IGF‐I were predominantely seen in ductal regions, suggest that IGF‐I may be a target of autoimmunity in Sjögren's syndrome.
OBJECTIVES: The purpose of this study was to investigate immunohistochemically the expression of tumor‐associated glycoprotein 72 (TAG‐72) using the monoclonal antibody (MAb) CC49 in salivary gland neoplasia and normal salivary glands in an attempt to determine the potential usefulness of MAb CC49 in diagnostic and therapetic applications.
MATERIALS AND METHODS: Eighty‐six specimens (21 benign tumors, 41 malignant, and 24 normal salivary glands), fixed in 10% formalin and embedded in paraffin, were retrieved from the files of the Department of Oral Medicine and Oral Pathology at the Dental School of Aristotle University, Thessaloniki, Greece, and were retrospectively studied with hematoxylin and eosin and with the streptavidin‐biotin‐complex method using the MAb CC49.
RESULTS: Strong immunoreactivity for TAG‐72 was observed in salivary duct carcinoma, adenocarcinoma, papillary cystadenocarcinoma, low‐grade mucoepidermoid carcinoma, normal submandibular, sublingual, and minor salivary glandS. Weak or no immunoreactivity was found in adenoid cystic carcinoma, basal cell adenocarcinoma, polymorphous low‐grade adenocarcinoma, and normal parotid gland.
CONCLUSIONS: Our results suggest the potential use of MAb CC49 in the differential diagnosis of some salivary gland neoplasms in which their histopathologic features overlap, and in the radiation immunolocalization and immunotherapy of malignant tumors that are localized in the parotid gland.
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