Phytochemical screening and antioxidant analysis are essential standardization procedure for the agarwood tea product. Gyrinops tea is an agarwood tea that has been emerged on Lombok Island recently. This research aims to carry qualitative phytochemical analysis and antioxidant activity assay of Gyrinops tea from several agarwood plantations on Lombok Island. Gyrinops versteegii leaves as the raw material of Gyrinops tea were taken from several agarwood plantations on Lombok Island, including Lingsar, Rarung, Kekait, Pejaring, and Mataram. There are three steps on G. versteegii leaves processing method into Gyrinops tea, including sterilization, drying, and chopping. G. versteegii leaves were brewed in distilled water at 70°C for 5 minutes to make Gyrinops tea. Phytochemical screening of Gyrinops tea was conducted with a qualitative assay identification of Flavonoid, Alkaloid, Saponin, and Tannin. The antioxidant assay of Gyrinops tea was investigated based on DPPH radicals scavenging activity. The result shows that Gyrinops tea positively contains tannin in high dosage and Flavonoid in a moderate dose with no alkaloid and saponin. Gyrinops tea samples have various antioxidant activity from moderate to strong based on IC50 measurement. It could be concluded that different sampling location has affected antioxidant activity of Gyrinops tea.
Agar wood farmer of Pejaring Village have used traditional method to induce resin formation on agarwood tree. They used mechanical method induction by nail and machete tools as well as chemical method induction by chemical liquid that the called by “infus”. These traditional methods influenced resin formation on agarwood tree at their agarwood plantation. That resin production was low in productivity and low in efficiency. The purpose of this community service was to conducted workshop of bio-induction technology for Pejaring Village agarwood farmer to solve their problem. Agarwood farmer of Pejaring Village were directly involved in all bio-induction training activity. This training was started by socialization of program through FGD with Agarwood Farmer of Pejaring Villange. Practical stage was started by selecting agarwood tree for sample by themselves. After selecting process has been done, training continued by bio-induction of agarwood sample using bore and injecting method also by themselves. The last stage was monitoring and evaluating of resin production on agarwood sample as result of bio-induction. In this stage, they got opportunity to give self-assessment of those resin production by organoleptic test. Bio-induction technology could stimulate resin formation on agarwood tree sample at 3 months after induction. This result was much better comparing to the result of traditional induction method that they used to conducted. Organoleptic test has confirmed that resin has quite good fragrance quality. It could be concluded that Pejaring Village agarwood farmers have understood about bio-induction technique and could applied that technology to replace traditional technology.
Abstract. Wangiyana IGAS, Supriadi, Nikmatullah A, Sunarpi, Triandini IGAAH. 2021. Diversity of Gyrinops versteegii from several agarwood plantation on Lombok Island (Indonesia) as raw material of Gyrinops tea. Biodiversitas 23: 178-186. The purpose of this research is to examine the diversity of Gyrinops versteegii as a raw material of agarwood Gyrinops tea based on morphology, phytochemical, and molecular characters. G. versteegii samples were taken from 5 agarwood plantation on Lombok Island: Lingsar, Rarung, Mataram, Kekait, and Pejaring. Variation of G. versteegii stem, leaves, and fruit was the morphology character observed in this study. Qualitative phytochemical screening, quantitative tannin concentration, and the hedonic score of Gyrinops tea were the essential phytochemical character in this study. DNA fingerprinting and RAPD analysis were the molecular characters of this study. All of those characters were used in numeric-phenetics analysis to construct a dendrogram. MVSP program with UPGMA algorithm as clustering method and Simple Matching Coefficient for similarity analysis was used to construct dendrogram. The result has shown variation topology of dendrogram based on morphology, phytochemical, molecular, and combination character. However, all dendrograms had the same number of clusters and cluster members. G. versteegii kekait and G. versteegii Pejaring were grouped to cluster 1 while G. versteegii Rarung and G. versteegii Mataram were grouped to cluster 2. G. versteegii Lingsar was grouped later on the node after cluster 1 or 2. It could be concluded that G. versteegii from Lombok Island Plantation could be divided into three different cluster groups based on the variation of morphology, chemical, and molecular characters.
The aims of this research are to apply Senaru forest rhizosphere on Kabupaten Lombok Barat West Nusa Tenggara as Mycorrhiza inoculants for Gyrinops versteegii germination and growth. Rhizosphere sample was taken from ten sampling spot on Senaru forest between Latitude: 08o18.808’ S – 08o19.174’ S and Longitude: 116o24.138’ E – 116o24.181’E. This study employed Factorial Experiment Design with 2 Factor including: Medium Composition (M) and Mychorizza Inoculant (I). There were 5 media composition: M1 (sand), M2 (soil:sand = 1:2), M3 (soil:sand = 1:1), M4 (soil:sand = 2:1), M5 (soil). There were 2 types of Inoculation: I1 (without inoculant) and I2 (innoculant from senaru Rhizosphere). Growth parameters observed in this study were: germination percentage, stem length, stem diameter and root colonization. Germination percentage of G. versteegii seeds in all growth media are below 60 % which could be classified as low germination rate. Also germination from media without rhizosphere is higher than germination from media with rhizosphere. On the other hand, G. versteegii growth on rhizosphere media is slightly higher than growth of G. versteegii on media without rhizosphere based on stem diameter and length measurement. It tends that medium composition with higher sand proportion tended to gives better germination and growth rate of G. verteegii. Myorrhiza colonization on G. versteegii root was higher in media with rhizosphere addition. It could be concluded that Application of Senaru rhizosphere containing Mycorrhiza increases G. versteegii growth but not its germination percentage. This research enrich knowledge in biological science about asociation of mycorrhiza with G. versteegii especially on its growth and germination
Tannin is one of the essential components that determine the quality of herbal tea products. This research aims to conduct a quantitative tannin assay of Gyrinops tea with different leaf sampling locations and processing methods. Factorial Completely Randomized Design was used as an experimental design with two treatment factors. The first factor is sampling location (Kekait, Lingsar, and Pejaring). The second factor is the processing method (Fresh leaves and Dried leaves). A titrimetric method with KMnO4 and Indigo carmine reagent was used for quantitative tannin measurement. ANOVA and DMRT at a 5% significant level were used for tannin concentration analysis. The result indicated that the first, second, and interaction factors significantly affected tannin concentration. Drying the G. versteegii leaves could increase the tannin concentration of the Gyrinops tea product. G. versteegii leaves taken from Kekait have shown highest tannin concentration among other regions. It could be concluded that dried G. versteegii leaves Kekait could produce Gyrinops tea with high quality based on tannin concentration measurement.
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