Lobelia inflata L. contains piperidine alkaloids. The main alkaloid is the pharmacologically active lobeline. We have studied the effect of macroelements (Mg 2+ , Na + and Ca 2+) on the growth and alkaloid production of hairy root cultures of L. inflata (clone 8009/f4). Macroelements influenced characteristically the linear growth and biomass formation of hairy roots. The greatest biomass formed in B5 media containing 1000 mg/l MgSO 4. It was found that the highest amount of lobeline (39 µg/g, dry weight) was reached at 75 mg/l CaCl 2. The results suggest that the optimal nutrient mixture for the best lobeline production was the basal B5 medium (containing 150 mg/l CaCl 2, 150 mg/l NaH 2 PO 4) supplemented with 1000 mg/l MgSO 4.
In vitro cultures of Atropa belladonna L. (Solanaceae) cultivated on a half dilution MS (Murashige and Skoog) medium containing 3% sucrose, was infected by microinjection with Agrobacterium rhizogenes strain R1601. The hairy roots formed within 2 months were isolated, subcultured on B 5 (Gamborg) medium and selected from the point of view of growth and tropane alkaloid production. Determination of alkaloids was performed by HPLC methods. Atropine, scopolamine and 6-OHhyoscyamine were identified and measured by this technique. We have studied the effect of MgSO 4 on the growth and alkaloid production of genetically modified hairy roots as well.
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