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In vitro cultures of Atropa belladonna L. (Solanaceae) cultivated on a half dilution MS (Murashige and Skoog) medium containing 3% sucrose, was infected by microinjection with Agrobacterium rhizogenes strain R1601. The hairy roots formed within 2 months were isolated, subcultured on B 5 (Gamborg) medium and selected from the point of view of growth and tropane alkaloid production. Determination of alkaloids was performed by HPLC methods. Atropine, scopolamine and 6-OHhyoscyamine were identified and measured by this technique. We have studied the effect of MgSO 4 on the growth and alkaloid production of genetically modified hairy roots as well.