Effect of stocking density on growth, production and survival of red tilapia (Oreochromis mossambicus × O. niloticus) were conducted in hapa for 60 days at Chilya Thatta. Tilapia fry (4.5±0.02 cm, 2.0±0.01 g) were stocked into hapa (4.5x2.4x1 m). Three treatments with two replicates were used: T 1 -200; T 2 -250 and T 3 -300 fry/hapa. Fry were fed twice a daily with pelleted feed containing 35% protein, 5% of total biomass. After 60 days, the highest growth were determined in T 1 (49.8±0.0 g) while in T 3 lowest growth (29.09±0.0 g) was recorded. Production (9.56, 10.13 and 8.12 kg/m 3 /60days) in T 1 , T 2 and T 3 respectively were significantly different (P<0.05) to each other. Feed conversion ratio of 0.48, 0.48 and 0.49 in T 1 , T 2 and T 3 , respectively not significantly different (P>0.05). Survival was significantly different among treatments (P<0.01). Highest survival (100%) was attained in T 1 with lower stocking density, followed by T 2 (98%) and T 3 (95%). Water quality parameters recorded throughout the study period were found within the ranges for fish culture such as temperature 27.2 to 28.5 °C, dissolved oxygen 5.8 to 6.4 mg/l, pH 6.9 to 7.6, ammonia from 0.45 to 0.51 mg/l, hardness 106 to 110 ppm and nitrite 0.151 to 0.162 mg/l.
Effect of stocking density on growth, production and survival of red tilapia (Oreochromis mossambicus × O. niloticus) were conducted in hapa for 60 days at Chilya Thatta. Tilapia fry (4.5±0.02 cm, 2.0±0.01 g) were stocked into hapa (4.5x2.4x1 m). Three treatments with two replicates were used: T1-200; T2-250 and T3-300 fry/hapa. Fry were fed twice a daily with pelleted feed containing 35% protein, 5% of total biomass. After 60 days, the highest growth were determined in T1 (49.8±0.0 g) while in T3 lowest growth (29.09±0.0 g) was recorded. Production (9.56, 10.13 and 8.12 kg/m3/60days) in T1, T2 and T3 respectively were significantly different (P<0.05) to each other. Feed conversion ratio of 0.48, 0.48 and 0.49 in T1, T2 and T3, respectively not significantly different (P>0.05). Survival was significantly different among treatments (P<0.01). Highest survival (100%) was attained in T1 with lower stocking density, followed by T2 (98%) and T3 (95%). Water quality parameters recorded throughout the study period were found within the ranges for fish culture such as temperature 27.2 to 28.5 °C, dissolved oxygen 5.8 to 6.4 mg/l, pH 6.9 to 7.6, ammonia from 0.45 to 0.51 mg/l, hardness 106 to 110 ppm and nitrite 0.151 to 0.162 mg/l.
To study the early molecular immunity of Echinococcus granulosus infection in livestock, especially in sheep, the immunohistochemistry, in-situ hybridization was used to study the relationship and the distribution between miR-216b and IL2RB in the intestine tissue of kazakh sheep with resistant and non-resistant MHC haplotypes after oral infection with E. granulosus eggs. The results demonstrated that miR-216b negatively regulated IL2RB in the cystic echinococcosis intestinal tissues, and they were differentially expressed in the intestine tissue between the resistant sheep and non-resistant sheep, more widely distributed in the resistant group. These results illustrated that miR-216b and IL2RB activated stronger immune response in the resistant sheep, compared with the non-resistant sheep, and participating in the T cell immune response.
Authors' Contribution RDB and ABK conceived and designed the experiments, performed experiments, interpreted the data and wrote the article. All other authors helped in preparation of the manuscript.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.