No abstract

SummaryTamoxifen has been implicated as a risk factor for venous thrombosis in advanced breast cancer although the evidence for increased arterial or venous thrombosis with tamoxifen in early breast cancer is less clear. The effect of tamoxifen on haemostasis, and thereby possible thromboembolic risk, was investigated in normal women enrolled in a placebo controlled trial of tamoxifen as a chemopreventative agent for breast cancer. There was an initial reduction in fibrinogen levels in all women on tamoxifen over the first year of follow-up and a marginal reduction in antithrombin III and Protein S in postmenopausal women at 6 months. There were no changes in cross linked fibrinogen degradation products or Protein C for pre or postmenopausal women. There was no increase in the incidence of thromboembolic events on tamoxifen. This study demonstrates that tamoxifen has only marginal effects on factors involved in haemostasis reported to affect the incidence of arterial or venous thromboembolic disease. The follow-up time is relatively short (maximum 36 months) and careful long term follow-up is necessary to detect clinically significant morbidity.

No abstract

Size-exclusion chromatography (SEC) of sodium acetate buffer, denatured isoamylase, and debranched starch is used with various NaCl concentration as mobile phase to identify and minimize interference of buffer and isoamylase in analysis of debranched starch molecular structure. Also, superdex (SD) 75, SD 75 + SD 30, or SD 200 + SD 30 is used to compare SEC results under different columns. When a deionized water is used as mobile phase, elution times of each buffer and isoamylase are overlapped with molecular distribution of debranched starch in all column conditions. Higher NaCl concentration of mobile phase delays elution time of isoamylase in all column conditions but does not change fractionation behavior of debranched starch chains. Elution time of isoamylase is overlapped with that of debranched starch at higher than 200 mM of NaCl concentration with SD 75. In SD 75 + SD 30 and SD 200 + SD 30 column, elution of isoamylase completely separate with that of debranched starch when NaCl concentration of mobile phase is higher than 50 mM. Overall, the interference is minimized by addition of NaCl to mobile phase with SD 75 + SD 30 and SD 200 + SD 30 column.