Flame transfer function measurements of turbulent premixed flames were made in a model lean premixed, swirl-stabilized, gas turbine combustor. OH*, CH*, and CO2* chemiluminescence emissions were measured to determine heat release oscillation from a whole flame, and the two-microphone technique was used to measure inlet velocity fluctuation. 2-D CH* chemiluminescence imaging was used to characterize the flame shape: the flame length (LCH* max) and flame angle (α). Using H2-natural gas composite fuels, XH2 = 0.00 ∼ 0.60, very short flame was obtained and hydrogen enrichment of natural gas had a significant impact on the flame structure and flame attachment points. For a pure natural gas flame, the flames exhibit a “V” structure, whereas H2-enriched natural gas flames have an “M” structure. Results show that the gain of “M” flames is much smaller than that of “V” flames. Similar to results of analytic and experimental investigations on the flame transfer function of laminar premixed flames, it shows that the dynamics of a turbulent premixed flame is governed by three relevant parameters: the Strouhal number (St = LCH* max / Lconv), the flame length (LCH* max), and the flame angle (α). Two flames with the same flame shape exhibit very similar forced responses, regardless of their inlet flow conditions. This is significant because the forced flame response of a highly turbulent, practical gas turbine combustor can be quantitatively generalized using the non-dimensional parameters which collapse all relevant input conditions into the flame shape and the Strouhal number.
Flame transfer function measurements of turbulent premixed flames are made in a model lean-premixed, swirl-stabilized, gas turbine combustor. OH∗, CH∗, and CO2∗ chemiluminescence emissions are measured to determine heat release oscillation from a whole flame, and the two-microphone technique is used to measure inlet velocity fluctuation. 2D CH∗ chemiluminescence imaging is used to characterize the flame shape: the flame length (LCH∗ max) and flame angle (α). Using H2-natural gas composite fuels, XH2=0.00–0.60, a very short flame is obtained and hydrogen enrichment of natural gas is found to have a significant impact on the flame structure and flame attachment points. For a pure natural gas flame, the flames exhibit a “V” structure, whereas H2-enriched natural gas flames have an “M” structure. Results show that the gain of M flames is much smaller than that of V flames. Similar to results of analytic and experimental investigations on the flame transfer function of laminar premixed flames, it shows that the dynamics of a turbulent premixed flame is governed by three relevant parameters: the Strouhal number (St=LCH∗ max/Lconv), the flame length (LCH∗ max), and the flame angle (α). Two flames with the same flame shape exhibit very similar forced responses, regardless of their inlet flow conditions. This is significant because the forced flame response of a highly turbulent, practical gas turbine combustor can be quantitatively generalized using the nondimensional parameters, which collapse all relevant input conditions into the flame shape and the Strouhal number.
A novel bacteriophage, PBES 02, infecting Cronobacter sakazakii was isolated and characterized. It has a spherical head of 90 nm in diameter and a tail of 130 nm in length, and belongs to Myoviridae as observed under a transmission electron microscope. The major virion protein appears to be 38 kilodaltons (kDa) in size. The latent period of PBES 02 is 30 min and the burst size is 250. Infectivity of the phage remained intact after exposure to temperatures ranging from 4°C to 55°C for 1 h. It was also stable after exposure to pHs ranging from 6 to 10 for 1 h. The phage effectively removed contaminating Cronobacter sakazakii from broth infant formula. PBES 02 has a double-stranded DNA genome of 149,732 bases. Its GC ratio is 50.7%. Sequence analysis revealed that PBES 02 has 299 open reading frames (ORFs) and 14 tRNA genes. Thirty-nine ORFs were annotated, including 24 related to replication and regulation functions, 10 related to structural proteins, and 5 related to DNA packaging. The genome of PBES 02 is closely related to that of two other C. sakazakii phages, CR3 and CR8. Comparison of DNA sequences of genes encoding the major capsid protein revealed a wide geographical distribution of related phages over Asia, Europe, and America.
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