A Newly Isolated Bacteriophage, PBES 02, Infecting Cronobacter sakazakii

Lee, Hyung Ju; Kim, Wan Il; Kwon, Young Chan; Eun, Kyung; Kim, Minjin; Myung, Heejoon

doi:10.4014/jmb.1605.05020

Cited by 18 publications

(14 citation statements)

References 23 publications

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“…After incubation, lysis zones were examined. The lytic activity of the phage was classified as clear (+) or no (–) reaction ( Lee et al, 2016 ).…”

Section: Methodsmentioning

confidence: 99%

Genetic Analysis and Characterization of a Bacteriophage ØCJ19 Active against Enterotoxigenic Escherichia coli

Kim¹,

Kim²,

Kim³

et al. 2020

Food Sci Anim Resour

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Enterotoxigenic Escherichia coli (ETEC) is the major pathogenic E. coli that causes diarrhea and edema in post-weaning piglets. In this study, we describe the morphology and characteristics of ØCJ19, a bacteriophage that infects ETEC, and performed genetic analysis. Phage ØCJ19 belongs to the family Myoviridae . One-step growth curve showed a latent phase of 5 min and burst size of approximately 20 phage particles/infected cell. Phage infectivity was stable for 2 h between 4°C and 55°C, and the phage was stable between pH 3 and 11. Genetic analysis revealed that phage ØCJ19 has a total of 49,567 bases and 79 open reading frames (ORFs). The full genomic sequence of phage ØCJ19 showed the most similarity to an Escherichia phage, vB_EcoS_ESCO41. There were no genes encoding lysogeny, toxins, virulence factors, or antibiotic resistance in this phage, suggesting that this phage can be used safely as a biological agent to control ETEC. Comparative genomic analysis in terms of the tail fiber proteins could provide genetic insight into host recognition and the relationship with other coliphages. These results showed the possibility to improve food safety by applying phage ØCJ19 to foods of animal origin contaminated with ETEC and suggests that it could be the basis for establishing a safety management system in the animal husbandry.

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“…After incubation, lysis zones were examined. The lytic activity of the phage was classified as clear (+) or no (–) reaction ( Lee et al, 2016 ).…”

Section: Methodsmentioning

confidence: 99%

Genetic Analysis and Characterization of a Bacteriophage ØCJ19 Active against Enterotoxigenic Escherichia coli

Kim¹,

Kim²,

Kim³

et al. 2020

Food Sci Anim Resour

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“…Brain heart infusion (BHI, Becton Dickinson, USA) broth and agar were used to culture the strain. Standard double-layer agar technique [21] was employed to generate a single plaque and calculate phage concentration.…”

Section: Host Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

“…The experiment was conducted three times. The results are reported as the mean of three observations ± standard deviation [12].…”

Section: Temperature and Ph Stability Of Phage Infectivitymentioning

confidence: 99%

“…The lysis activity of the phage was classified as clear (+) and no reaction (-). For hostrange testing, Enterobacter and other strains were obtained from KCTC (Korean Collection for Type Cultures) or ATCC (American Type Culture Collection) [12].…”

Section: Host-range Testmentioning

confidence: 99%

“…In 2006, the US Food and Drug Administration (FDA) approved the use of purified bacteriophages as food additives [11]. In a previous study, the complete genomic sequence of a bacteriophage infecting C. sakazakii was reported, and phage genome analysis revealed no toxicityrelated genes encoding toxic agents and toxins [11][12][13][14][15][16][17][18]. The use of bacteriophages as biological agents is a potential alternative to the use of antibiotics [19].…”

Section: Introductionmentioning

confidence: 99%

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Characterization and Genomic Analysis of Novel Bacteriophage ��CS01 Targeting Cronobacter sakazakii

Kim

et al. 2019

Journal of Microbiology and Biotechnology

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Cronobacter sakazakii is an opportunistic pathogen causing serious infections in neonates. In this study, a bacteriophage ΦCS01, which infects C. sakazakii, was isolated from swine feces and its morphology, growth parameters, and genomic analysis were investigated. Transmission electron microscopy revealed that ΦCS01 has a spherical head and is 65.74 nm in diameter with a 98.75 nm contracted tail, suggesting that it belongs to the family Myoviridae. The major viral proteins are approximately 71 kDa and 64 kDa in size. The latent period of ΦCS01 was shown to be 60 min, and the burst size was 90.7 pfu (plaque-forming units)/ infected cell. Bacteriophage ΦCS01 was stable at 4-60°C for 1 h and lost infectivity after 1 h of heating at 70°C. Infectivity remained unaffected at pH 4-9 for 2 h, while the bacteriophage was inactivated at pH <3 or >10. The double-stranded ΦCS01 DNA genome consists of 48,195 base pairs, with 75 predicted open reading frames. Phylogenetic analysis is closely related to that of the previously reported C. sakazakii phage ESP2949-1. The newly isolated ΦCS01 shows infectivity in the host bacterium C. sakazakii, indicating that it may be a promising alternative to antibacterial agents for the removal of C. sakazakii from powdered infant formulas.

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Genomic Characterization of Twelve Lytic Bacteriophages Infecting Midgut Bacteria of Aedes aegypti

et al. 2022

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A Newly Isolated Bacteriophage, PBES 02, Infecting Cronobacter sakazakii

Cited by 18 publications

References 23 publications

Genetic Analysis and Characterization of a Bacteriophage ØCJ19 Active against Enterotoxigenic Escherichia coli

Genetic Analysis and Characterization of a Bacteriophage ØCJ19 Active against Enterotoxigenic Escherichia coli

Characterization and Genomic Analysis of Novel Bacteriophage ��CS01 Targeting Cronobacter sakazakii

Genomic Characterization of Twelve Lytic Bacteriophages Infecting Midgut Bacteria of Aedes aegypti

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