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Frequency and severity of side effects during treatment of acute oral infection by T. cruzi demand direct supervision and close follow-up, even in those asymptomatic, to prevent life-threatening situations.

Extraction performance of the liquid-liquid batch (LLB) and continuous extraction, as well as simultaneous distillation-extraction were evaluated in order to analyze volatile compounds in tequila. Initially, the best extraction conditions were obtained for each method using tequila samples. Conditions tested were solvent type, extraction time, sample's alcoholic concentration and, for the LLB, the number of successive extractions. Response variables were chromatograms, number of peaks, and total area. In a second phase, its extraction performance was evaluated with a model solution of 38 compounds. In both steps, obtained extracts were analyzed by gas chromatography. The best results were obtained with the LLB method since it makes possible to recover a good number of volatile compounds in few minutes. Additionally, this method showed the best performance after extraction and concentration, and the lower CV.Keywords: aroma; flavor; batch liquid-liquid; continuous liquid-liquid; simultaneous extraction-distillation; tequila Se evaluo´el desempen˜o de tres me´todos extractivos (extraccio´n lı´quido-lı´quido por lotes y en continuo y extraccio´n-destilacio´n simulta´nea) seleccionados para analizar vola´tiles de tequila. Inicialmente se obtuvieron las mejores condiciones de extraccio´n para cada me´todo utilizando muestras de tequila. Las condiciones evaluadas fueron el tipo de solvente, tiempo de extraccio´n, concentracio´n alcoho´lica de la muestra y para el me´todo lı´quido-lı´quido por lote, nu´mero de extracciones sucesivas. Las variables de respuesta fueron el nu´mero de picos y el a´rea total en los cromatogramas. En una segunda etapa, se evaluo´su desempen˜o de extraccio´n con una solucio´n modelo de 38 compuestos. En ambas fases, los extractos fueron analizados por cromatografı´a de gases. Los mejores resultados se obtuvieron con el me´todo lı´quido-lı´quido por lote, ya que e´ste permite recuperar un buen nu´mero de compuestos en pocos minutos. Adicionalmente, este me´todo mostro´el mejor desempen˜o despue´s de extraccio´n y concentracio´n asıć omo el menor CV. Palabras