This paper considers the problem of non-preemptive hard realtime scheduling of sporadic task graphs on a uniprocessor with Earliest Deadline First (EDF) scheduling. A sporadic task graph associated to an application is characterized by a set of subtasks under precedence constraints with deadline constraints. We study feasibility conditions based on the computation of the worst case response times of any subtask of a task graph. We investigate the late deadline constraint.
In this study, a rapid, simple, highly sensitive and anti-interference method for the joint detection of four IgG subtypes was established by using Raman microspheres with four characteristic Raman spectra. The results showed that the concentrations of IgG1 in the range of 0-1500ng/mL, IgG2 in the range of 0-1100ng/mL, IgG3 in the range of 0-88.7ng/mL, IgG4 in the range of 0-77.2ng/mL showed a good correlation with the response value of The Raman signal. The lowest detection limits were 25.4ng/mL, 21.7ng/mL, 1.6ng/mL, 1.7ng/mL, respectively. Reproducibility is good, the CV of low, medium and high concentration standard solution was within 10%. The recoveries of four IgG subtypes were in the range of 90%-110%, and the accuracy of the method was good. The coefficients of variation between and within the three batches of reagents were all less than 11%, showing good precision. There was no cross reaction with PCT (20ng/ mL), IL-6(1ng/ mL) and BSA (10mg/ mL), and the specificity was good. Common interfering substances such as bilirubin, triglyceride and trisodium citrate did not affect the determination results, and heparin sodium only affected the determination results of IgG1. This method has good anti-interference ability. The method has high sensitivity, simple operation and strong anti-interference ability. It has good correlation with the IgG detection methods commonly used in clinic and can be used for rapid and quantitative detection of four immunoglobulin G subtypes.
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