A two-step fermentation procedure for maize straw hydrolysis by Phanerochaete chrysosporium, Trichoderma and Aspergillus nigerwere investigated in this study. 2 mL of P. chrysosporium (107spores/mL) wasadded to 50 mL medium for the first fermentation stage. The optimal culture conditions were 28°C of culture temperature, 4.0 of pH, 8 days of culture time, 2 mL of Trichoderma suspensions (107spores/mL) and 1 mL of A. niger suspensions (107spores/mL) of the inoculums size on the second fermentation stage. Under the optimal conditions, the crude degradation rate achieved 48.2%. Via sulfonation, oxidate-lignosulfonate alkali lignin (OLAL) was achieved from maize straw hydrolysate(MSH).OLAL and MSH can be partial substitution for common water reducers and OLAL was more efficient compared to that of MSH.
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