Two novel meso-CF3 BODIPY-based fluorescent rotors have been rationally prepared and sensitively respond to viscosity in living cells with fluorescence “turn-on” effect, attributing to the special restricted rotation of meso-CF3...
β-Amyloid
deposition is one of the main pathological features
of Alzheimer’s disease (AD). The development of fluorescent
probes targeting specific β-amyloid species has recently become
an attractive strategy to achieve the early diagnosis of AD. In this
work, a dual-channel fluorescent protein chromophore derivative C17 was rationally designed and synthesized for the detection
and discrimination of Aβ42 aggregates and oligomers. C17 exhibits a specific turn-on emission peak for Aβ42 oligomers at ∼470 nm (peak A) and a peak at ∼600
nm (peak B) for both Aβ42 oligomers and Aβ42 aggregates. Taking advantage of the dual emission of the
probe, the dynamic aggregation process of the Aβ42 peptide was monitored in solution. Moreover, double staining of
brain sections from transgenic AD mice revealed that peak A of C17 preferentially detected Aβ42 oligomers,
whereas peak B was more sensitive to Aβ42 aggregates.
The fact that probe C17 can be used for dissecting these
two Aβ42 species makes C17 a comprehensive
tool for β-amyloid aggregation studies in AD research.
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