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A B S T R A C TIn this work, novel nanoflower/alginate microbeads were synthesized to immobilize α-acetolactate decarboxylase (ALDC) with a facile approach. Typically, ALDC was first coprecipitated with Ca 3 (PO 4 ) 2 to form enzymeinorganic hybrid nanoflowers (Ca 3 (PO 4 ) 2 -ALDC), and then the nanoflowers containing micrometre-sized particles and nanoscale flower-like petals were entrapped in alginate gel beads (Ca 3 (PO 4 ) 2 -ALDC) @Alg). Compared with free ALDC and Ca 3 (PO 4 ) 2 -ALDC nanoflowers, the microbeads performed greatly improved recyclability and stability, meanwhile, retained 98% of activity compared with free ALDC. Moreover, the immobilized enzyme can be used in a 300 L beer fermentation tank to prevent the formation of diacetyl, solve the problem of beer off-flavor and short the beer maturation time, thus exhibiting a great potential application in beer brewing industry.

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