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12In the present study, multicomponent competitive biosorption of heavy metal from aqueous 13 solution onto pretreated dried Aspergillus niger in batch system was investigated. The 14 adsorption data were fitted to the multicomponent Langmuir, Freundlich, Temkin and Sips 15 equations. We used the genetic algorithm of biosorption in ternary mixture to evaluate the 16 potential effects of each metal in the removal of other metals. In order to take both 17 mechanisms of the cell-surface binding and intra-particle diffusion into account, an 18 alternative model was investigated by combining the pseudo-second-order kinetics model and 19 the intra-particle diffusion model. A model describing the process of biosorption by a single-20 stage batch design was developed and verified based on the Temkin isotherm model. 21 Fundamentally, the outlook from these observations of the experiments that the pretreated 22 dried biomass is a suitable absorbent for the removal of significant amounts of the heavy 23 metal from the effluents of industrial wastewater is promising.24 25

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