A highly reproducible plant electrical signal–light-induced bioelectrogenesis (LIB) was obtained by means of periodic illumination/darkness stimulation of broad bean (Vicia faba L.) leaves. By stimulating the same position of the same leaf with different concentrations of NaCl, we observed that the amplitude and waveform of the LIB was correlated with the intensity of stimulation. This method allowed us to link dynamic ion fluxes induced by periodic illumination/darkness to salt stress. The self-referencing ion electrode technique was used to explore the ionic mechanisms of the LIB. Fluxes of H+, Ca2+, K+, and Cl− showed periodic changes under periodic illumination/darkness before and after 50 mM NaCl stimulation. Gray relational analysis was used to analyze correlations between each of these ions and LIB. The results showed that different ions are involved in surface potential changes at different stages under periodic illumination/darkness. The gray relational grade reflected the contribution of each ion to the change in surface potential at a certain time period. The ion fluxes data obtained under periodic illumination/darkness stimulation will contribute to the future development of a dynamic model for interpretation of electrophysiological events in plant cells.
Gamma-aminobutyric acid (GABA), a four-carbon non-protein amino acid, is a significant component of the free amino acid pool, there are numerous reports that rapid and large increases in GABA levels occur in plants, in response to a variety of biotic and abiotic stress. Based on its metabolism and putative roles in plants, GABA is considered a natural chemical to increase wheat salt-tolerance. So this study investigated the exogenous GABA on wheat seedling (Triticum aestivum L. cv. Changwu134 and zhouyuan9369) growth and absorption of salt ions under normal or salt-stressed conditions. The results demonstrated that salt stress inhibited growth of wheat seedlings, decreased dry weight and water content, altered ion balance within the stressed seedlings. Pretreatment with 50mg/L GABA increased seedling biomass and K + content in leaves, decreased Na + content in leaves and roots under salt-stressed conditions by improving Na + exclusion, K + retention. These results indicated that exogenous 50mg/L GABA improved seedling growth and alleviated the inhibition due to salt stress of wheat by altered ion balance. Exogenous GABA has the capability of restraining transportation of salt ions to leaves and sustaining normal function of leaves. And the effect of exogenous GABA is obvious in common variety (zhouyuan9369) than in salt-tolerance variety (changwu134).
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