Three-dimensional multicellular spheroids (MCSs) have received extensive attention in the field of biomedicine due to their ability to simulate the structure and function of tissues in vivo more accurately than traditional in vitro two-dimensional models and to simulate cell–cell and cell extracellular matrix (ECM) interactions. It has become an important in vitro three-dimensional model for tumor research, high-throughput drug screening, tissue engineering, and basic biology research. In the review, we first summarize methods for MCSs generation and their respective advantages and disadvantages and highlight the advances of hydrogel and microfluidic systems in the generation of spheroids. Then, we look at the application of MCSs in cancer research and other aspects. Finally, we discuss the development direction and prospects of MCSs

No abstract

Acoustic-based microfluidics has been widely used in recent years for fundamental research due to its simple device design, biocompatibility, and contactless operation. In this article, the basic theory, typical devices, and technical applications of acoustic microfluidics technology are summarized. First, the theory of acoustic microfluidics is introduced from the classification of acoustic waves, acoustic radiation force, and streaming flow. Then, various applications of acoustic microfluidics including sorting, mixing, atomization, trapping, patterning, and acoustothermal heating are reviewed. Finally, the development trends of acoustic microfluidics in the future were summarized and looked forward to.

With the growing demand for miniaturized workspaces, the demand for microrobots has been increasing in robotics research. Compared to traditional rigid robots, soft robots have better robustness and safety. With a flexible structure, soft robots can undergo large deformations and achieve a variety of motion states. Researchers are working to design and fabricate flexible robots based on biomimetic principles, using magnetic fields for cable-free actuation. In this study, we propose an inchworm-shaped soft robot driven by a magnetic field. First, a robot is designed and fabricated and force analysis is performed. Then, factors affecting the soft robot’s motion speed are examined, including the spacing between the magnets and the strength and frequency of the magnetic field. On this basis, the motion characteristics of the robot in different shapes are explored, and its motion modes such as climbing are experimentally investigated. The results show that the motion of the robot can be controlled in a two-dimensional plane, and its movement speed can be controlled by adjusting the strength of the magnetic field and other factors. Our proposed soft robot is expected to find extensive applications in various fields.