The 1.85 A crystal structure of endonuclease III, combined with mutational analysis, suggests the structural basis for the DNA binding and catalytic activity of the enzyme. Helix‐hairpin‐helix (HhH) and [4Fe‐4S] cluster loop (FCL) motifs, which we have named for their secondary structure, bracket the cleft separating the two alpha‐helical domains of the enzyme. These two novel DNA binding motifs and the solvent‐filled pocket in the cleft between them all lie within a positively charged and sequence‐conserved surface region. Lys120 and Asp138, both shown by mutagenesis to be catalytically important, lie at the mouth of this pocket, suggesting that this pocket is part of the active site. The positions of the HhH motif and protruding FCL motif, which contains the DNA binding residue Lys191, can accommodate B‐form DNA, with a flipped‐out base bound within the active site pocket. The identification of HhH and FCL sequence patterns in other DNA binding proteins suggests that these motifs may be a recurrent structural theme for DNA binding proteins.
Academics who teach upper-level biology courses at tertiary education institutions typically establish prerequisites for their courses, with the anticipation that students will arrive at class prepared with a good understanding of basic concepts of chemistry and biology. Establishing prerequisites serves two purposes: it justifies the omission of ?previously learned? material from the curriculum and also permits the assumption that students are prepared to move to higher-level learning domains and tackle conceptually complex topics.
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