1995
DOI: 10.1002/j.1460-2075.1995.tb00083.x
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Abstract: The 1.85 A crystal structure of endonuclease III, combined with mutational analysis, suggests the structural basis for the DNA binding and catalytic activity of the enzyme. Helix‐hairpin‐helix (HhH) and [4Fe‐4S] cluster loop (FCL) motifs, which we have named for their secondary structure, bracket the cleft separating the two alpha‐helical domains of the enzyme. These two novel DNA binding motifs and the solvent‐filled pocket in the cleft between them all lie within a positively charged and sequence‐conserved s… Show more

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Cited by 465 publications
(560 citation statements)
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“…A critical characteristic of such nucleases is thus their ability to bind to the relevant DNA substrate, and most exonucleases do so in a non-sequence specific manner, though they generally show some degree of structure specificity. For example, the 5′-3′ exo CTD superfamily share a common helix hairpin helix (HhH) motif (Doherty et al 1996;Thayer et al 1995) that permits broad specificity DNA binding, probably through hydrogen bonding between the sugar-phosphate backbone of DNA and the protein backbone.…”
Section: Introductionmentioning
confidence: 99%
“…A critical characteristic of such nucleases is thus their ability to bind to the relevant DNA substrate, and most exonucleases do so in a non-sequence specific manner, though they generally show some degree of structure specificity. For example, the 5′-3′ exo CTD superfamily share a common helix hairpin helix (HhH) motif (Doherty et al 1996;Thayer et al 1995) that permits broad specificity DNA binding, probably through hydrogen bonding between the sugar-phosphate backbone of DNA and the protein backbone.…”
Section: Introductionmentioning
confidence: 99%
“…Endonuclease III (Endo III) was the first glycosylase discovered to contain this metal cofactor [8]. Endo III removes a wide variety of oxidized pyrimidines from DNA and contains the helixhairpin-helix (HhH) recognition motif [11][12][13][14][15][16][17][18][19]. MutY, structurally similar to Endo III [18][19][20][21], is another BER glycosylase that contains a [4Fe-4S] cluster [20].…”
Section: Introductionmentioning
confidence: 99%
“…Endo III removes a wide variety of oxidized pyrimidines from DNA and contains the helixhairpin-helix (HhH) recognition motif [11][12][13][14][15][16][17][18][19]. MutY, structurally similar to Endo III [18][19][20][21], is another BER glycosylase that contains a [4Fe-4S] cluster [20]. However, MutY instead removes adenine from 8-oxo-guanine:adenine mispairs [22][23][24][25][26][27][28][29][30][31][32][33][34].…”
Section: Introductionmentioning
confidence: 99%
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“…Endonuclease III (EndoIII) is a [4Fe-4S] cluster containing DNA repair enzyme from the helix-hairpin-helix family of DNA glycosylases, crucial for removal of oxidation damaged bases in DNA. 3,4 Early evidence indicated that Fe-S clusters in these enzymes are not amenable to oxidation or reduction in solution and suggested structural or regulatory roles of the cofactor. 5,6 More recent electrochemical studies of glycosylases immobilised on DNA-modified electrodes indicated that the [4Fe-4S] cluster is activated towards oxidation upon binding to DNA.…”
mentioning
confidence: 99%