Green synthesis of nanoparticles which have eco-friendly favourable solvent systems and environmentally reducing agents is of excessive importance. In this paper, we aimed to develop environmentally friendly, rapid and simple producer for the synthesis gold nanoparticles (Au-NPs) using aqueous extract of sumac as reducing agents for gold ions as well capping agent for the bioformed Au-NPs. The bio-synthesized Au-NPs were characterized by the UV-visible spectroscopy, FTIR, TEM, and zeta-potential measurements. The surface plasmon resonance band centred at 520 nm for Au-NPs was characterized by UV-visible spectrophotometer. The probable bio-molecules are polyphenols may responsible for reduction of gold ions were recognized through FT-IR. The TEM result shows the bioformed Au-NPs are spherical in shapes with the mean size of 20.83 ±4.4 nm. The capping of anionic bio-molecules on the surface of Au-NPs was confirmed by zeta potential assessment (-25.3 mV) and is responsible for the electrostatic stability. In vitro antioxidant activity studies showed that DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2, 2'-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid) activities increased in a dose dependent manner. The bio-synthesized nanoparticles can potentially useful in pharmaceutical and biomedical applications.
A peptide from ostrich (Struthio camelus) egg white protein hydrolysate (OEWPH) was purified, characterized, and its antioxidant and enzyme inhibitory properties were evaluated. The OEWPH was prepared using pepsin and pancreatin, and then fractionated using reversed-phase high performance liquid chromatography. The antioxidant activity of the WG-9 peptide was investigated based on its scavenging capacity for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,20-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), superoxide (O), hydroxyl (OH), and lipid peroxidation inhibition. The angiotensin-converting enzyme (ACE) inhibitory activity and kinetic parameters of the peptide were determined using N-[3-(2-Furyl)acryloyl]-L-phenylalanyl-glycyl-glycine (FAPGG) as a substrate. Tandem mass spectrometry analysis of the purified peptide revealed a sequence of WESLSRLLG (MW: 1060 Da; WG-9). This peptide inhibited linoleic acid oxidation and acted as a DPPH (IC = 15 ± 0.4 μg/mL), ABTS (IC = 130 ± 4.5 μg/mL), superoxide (IC = 160 ± 6.4 μg/mL), and hydroxyl (IC = 150 ± 6.7 μg/mL) radical scavenger. The ACE-inhibitory activity and kinetic parameters of the WG-9 peptide were determined, showing an ACE inhibitory activity with IC of 46.7 ± 1.4 μg/mL. The parameters of peptide/ACE interactions were investigated by molecule docking. Furthermore, viability assays showed that the identified peptide had no cytotoxicity against an HFLF-PI-5 cell line. In conclusion, the WG-9 peptide showed potent antioxidant and ACE-inhibitory activity.
Gastric cancer (GC) as the fourth most common cause of malignancies shows high rate of morbidity appropriating the second leading cause of cancer-related death worldwide. Developmental pluripotency associated-2 (DPPA2), cancer-testis antigen (CT100), is commonly expressed only in the human germ line and pluripotent embryonic cells but it is also present in a significant subset of malignant tumors. To investigate whether or not DPPA2 expression is recalled in GC, our aim in this study was to elucidate DPPA2 protein expression in gastric cancer. Fifty five GC tumor and their related margin normal tissues were recruited to evaluate DPPA2 protein expression and its probable associations with different clinicopathological features of the patients. DPPA2 was overexpressed in GC cases compared with normal tissues (P < .005). While DPPA2 expression was detected in all GC samples, its high expression was found in 23 of 55 tumor tissues (41.8%). Interestingly, 50 of 55 normal samples (90.9%) were negative for DPPA2 protein expression and remained 5 samples showed very low expression of DPPA2. DPPA2 protein expression in GC was significantly correlated with lymph node metastasis (p = 0.012). The clinical relevance of DPPA2 in GC illustrated that high level expression of this protein was associated with lymph node metastasis supporting this hypothesis that alteration in DPPA2 was associated with aggressiveness of gastric cancer and may be an early event in progression of the disease. DPPA2 may be introduced as a new marker for invasive and metastatic GCs.
The aim of this study was to examine antioxidant and inhibitory activity of Gly‐Ala‐Ser‐Arg‐His‐Trp‐Tyr‐Phe‐Leu peptide named GL‐9 peptide. The synthetic GL‐9 peptide was purified by C8 reveres phase HPLC (RP‐HPLC). The antioxidant activity of the GL‐9 peptide was investigated based on its scavenging capacity1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical, 2,2′‐azinobis (3‐ethylbenzothiazoline‐6‐sulphonic acid) diammonium salt (ABTS), superoxide ( normalO2•−), hydroxyl (OH•−) and lipid peroxidation inhibition. The ACE inhibitory activity and kinetic parameters of the peptide were determined using FAPPG as substrate. GL‐9 peptide/ACE complex interaction, position, type and energy was also investigated by using molecular docking. GL‐9 peptide displays DPPH (IC50 = 6.5 µg/mL), ABTS•+ (IC50 = 7.2 µg/mL), superoxide (IC50 = 122 µg/mL) and hydroxyl (IC50 = 60.0 µg/mL) radical scavengers. GL‐9 peptide showed an IC50 value of 47.8 µg/mL which inhibited ACE activity. The results indicated that the GL‐9 peptide has antioxidant and ACE inhibitory capacity. Practical Applications Synthetic GL‐9 peptide antioxidant and anti‐hypertensive activity were investigated using in vitro assays. Results indicated that this peptide possesses a high potent antioxidant activity as well as ACE‐inhibitory activity, demonstrate its potential application as an ingredient in wholesome foodstuffs.
Aim The aim of study was synthesized both silver- and zinc-oxide nanoparticles utilizing the Peganum harmala smoke extract (PHSE) bio-platform to evaluate their cytotoxicity on different types of human cancer cell lines and study their antioxidant, and antiangiogenic potentials. Material and Methods The Ag and ZnO nanoparticles were produced utilizing the green-synthesize method applying PHSE bio-platform. After characterization by XRD, DLS, FESEM, and FTIR methods, MTT assay was used for evaluation toxicity of nanoparticles. ABTS, DPPH, FRAP and ROS for antioxidant capacity, CAM and qPCR for antiangiogenesis effects of nanoparticle’s were used Results Ag-NPs (82.42 nm) and ZnO-NPs (163.05 nm) were inhibited prostate, ovarian and liver malignant cells. Inhibition ABTS•+ and DPPH•+ and increasing the rate of intracellular ROS exhibited the anti and pro-oxidant capacity of Ag and ZnO-NPs out and inside of malignant cells. Also, their anti-angiogenesis impact was verified by significant dose-dependent VEGF and VEGFR down-regulation and the decreased blood vessels in the chicks’ chorioallantoic membrane (CAM). Conclusion The antioxidant, cytotoxicity and anti-angiogenesis effects of Ag and ZnO-NPs synthesized from Pecan smoke extract make it possible to use these nanoparticles in cancer chemotherapy.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.