Putative mechanism for cancer suppression by PLGA nanoparticles loaded with<i>Peganum harmala</i>smoke extract

Shabestarian, Hoda; Tabrizi, Masoud Homayouni; Movahedi, Monireh; Neamati, Ali; Sharifnia, Fariba

doi:10.1080/02652048.2021.1917715

Cited by 19 publications

(11 citation statements)

References 35 publications

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“…It is worth mentioning that only very few studies have reported the anticancer activity of harmala using nanocarriers. For instance, a study reported the anticancer effect of P. harmala smoke-loaded poly(lactic- co -glycolic acid) (PLGA) nanoparticles …”

Section: Resultsmentioning

confidence: 99%

Peganum harmala Alkaloids and Tannic Acid Encapsulated in PAMAM Dendrimers: Improved Anticancer Activities as Compared to Doxorubicin

Azzazy

Sawy

Abdelnaser

et al. 2022

ACS Appl. Polym. Mater.

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pH-responsive dual-loaded nanoplatforms based on polyamidoamine dendrimer with a hydroxyl-terminated surface (PAMAM–OH), generations 3, 4, and 5 (G3, G4, and G5, respectively), were loaded with Peganum harmala alkaloid extracts (H) and tannic acid (TA) and tested against breast cancer cells. G3 dendrimers encapsulating the drugs showed the smallest sizes, PDI, and highest ζ-potential and entrapment efficiencies compared to those of G4 and G5. G3 dendrimers loaded with either doxorubicin (DOX), H, or TA or dual-loaded with H+TA were prepared. The average diameters of the designed DOX@G3, H@G3, TA@G3, and H+TA@G3 NPs were 78 ± 5.1, 122 ± 3.7, 141 ± 2.8, and 192 ± 1.9 nm, respectively. All of the prepared NPs exhibited high positive surface charges and high entrapment efficiencies. Release studies showed the ability of the dendrimers to release their cargo selectively in the cancerous cells’ extracellular pH (pH 5.5) via a pH-triggered mechanism while maintaining an outstanding stability at physiological conditions (pH 7.4). Furthermore, H@G3, TA@G3, and H+TA@G3 NPs exhibited remarkable cytotoxic activities against breast adenocarcinoma (MCF-7) cells with IC50 values of 2.6 ± 0.08, 4.5 ± 0.9, and 1.7 ± 0.6 μg/mL, respectively, as compared to free H, free TA, free DOX, and DOX@G3 (IC50 values of 5.8 ± 0.8, 7.2 ± 1, 6.75 ± 0.7, and 4.7 ± 0.8 μg/mL). The formulated H@G3, TA@G3, and DOX@G3 exhibited 11.8, 9.3, and 5.3% increases in late apoptosis in MCF-7 cells relative to their respective free forms, while H+TA@G3 exhibited the highest percentage of late apoptosis in MCF-7 cells. In conclusion, G3 PAMAM–OH dendrimers dually loaded with Peganum harmala alkaloids and tannic acid hold the potential for effective treatment of breast cancer.

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Section: Resultsmentioning

confidence: 99%

Peganum harmala Alkaloids and Tannic Acid Encapsulated in PAMAM Dendrimers: Improved Anticancer Activities as Compared to Doxorubicin

Azzazy

Sawy

Abdelnaser

et al. 2022

ACS Appl. Polym. Mater.

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“…Likewise, Aldawsari et al reported the antioxidant effects of resveratrol encapsulated in chitosan-coated PLGA-NPs in the H1299 lung carcinoma cell line (47). In their study, Shabestarian et al encapsulated Peganum harmala smoke extract in PLGA-NPs (48). Using ABTS, DPPH, and FRAP methods, they showed that these synthesized NPs had antioxidant effects (48).…”

Section: Discussionmentioning

confidence: 99%

“…In their study, Shabestarian et al encapsulated Peganum harmala smoke extract in PLGA-NPs (48). Using ABTS, DPPH, and FRAP methods, they showed that these synthesized NPs had antioxidant effects (48). In other studies, the PLGA-NPs were employed to encapsulate and enhance the antioxidant properties of natural compounds (49,50).…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory and Antioxidant Properties of PLGA Nanoparticles Produced From Kombucha Extract on A2780 Human Ovarian Cancer Cell Line

Ghandehari,

Homayouni Tabrizi,

Izadi Nia

et al. 2021

Avicenna J Med Biochem

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Background: Anti-cancer agents encapsulated in nanoparticles (NPs) can result in higher efficiency. Kombucha is a fermented tea beverage, and previous reports support its anti-cancer properties. Objectives: The present study aimed to evaluate the anti-cancer and anti-inflammatory properties of poly (lactic-co-glycolic acid) loaded Kombucha NPs (PLGA-K-NPs) against the A2780 human ovarian cancer cell line. Methods: The antioxidant activity was analyzed using ferric reducing ability of plasma and 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) assays, along with the measurement of catalase (CAT) gene expression. The gene expression of three interleukins (IL-1β, IL-6, and IL-10) was also determined to demonstrate the anti-inflammatory properties of NPs. Results: The results revealed the antioxidant effects of PLGA-K-NPs on the studied cell lines by increasing Fe3+ reduction, inhibiting the free radical formation (P<0.001), and increasing the expression of the CAT gene (P<0.001). In addition, NPs could significantly elevate the gene expression of IL-10 (P<0.01) as an anti-inflammatory cytokine at a 40 µg/mL concentration, while reducing the expression of IL-1β and IL-6, and inflammatory cytokines at all tested concentrations (P<0.01). Conclusion: According to the obtained results, PLGA-K-NPs have anti-inflammatory and anti-oxidant properties, therefore, they can be considered as a compound in the treatment of ovarian cancer. However, it needs to be further investigated in animal studies to clarify more details.

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“…Nanoparticles of various structures, especially polymeric ones, are the most promising platform for the creation of such DDSs [ 34 , 35 ]. PLGA polymer is one of the most widely used polymers in the development of nanoparticles as anticancer agents [ 36 , 37 , 38 , 39 , 40 ]. Laurel essential oil has been used as an antimicrobial, antioxidant [ 41 , 42 ] diuretic [ 43 ], and for the relief of rheumatic pains [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

Laurus nobilis L. Essential Oil-Loaded PLGA as a Nanoformulation Candidate for Cancer Treatment

et al. 2022

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The aim of this study was to obtain essential oil (LNEO) from the Laurus nobilis L. plant, and to prepare LNEO-loaded poly lactic-co-glycolic acid (PLGA) nanoparticles (NPs) as an approach in cancer treatment. The components of the obtained LNEO were analyzed using GC-MS. The LNEO-NPs were synthesized by the single-emulsion method. The LNEO-NPs were characterized using UV-Vis spectrometry, Dynamic Light Scattering (DLS), Scanning Electron Microscopy (SEM), and a DNA binding assay, which was performed via the UV-Vis titration method. According to the results, the LNEO-NPs had a 211.4 ± 4.031 nm average particle size, 0.068 ± 0.016 PdI, and −7.87 ± 1.15 mV zeta potential. The encapsulation efficiency and loading capacity were calculated as 59.25% and 25.65%, respectively, and the in vitro drug release study showed an LNEO release of 93.97 ± 3.78% over the 72 h period. Moreover, the LNEO was intercalatively bound to CT-DNA. In addition, the mechanism of action of LNEO on a dual PI3K/mTOR inhibitor was predicted, and its antiproliferative activity and mechanism were determined using molecular docking analysis. It was concluded that LNEO-loaded PLGA NPs may be used for cancer treatment as a novel phytotherapeutic agent-based controlled-release system.

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Putative mechanism for cancer suppression by PLGA nanoparticles loaded withPeganum harmalasmoke extract

Cited by 19 publications

References 35 publications

Peganum harmala Alkaloids and Tannic Acid Encapsulated in PAMAM Dendrimers: Improved Anticancer Activities as Compared to Doxorubicin

Peganum harmala Alkaloids and Tannic Acid Encapsulated in PAMAM Dendrimers: Improved Anticancer Activities as Compared to Doxorubicin

Anti-inflammatory and Antioxidant Properties of PLGA Nanoparticles Produced From Kombucha Extract on A2780 Human Ovarian Cancer Cell Line

Laurus nobilis L. Essential Oil-Loaded PLGA as a Nanoformulation Candidate for Cancer Treatment

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