BACKGROUND: We examined plasma microRNA (miRNA) concentrations from patients with gastric cancers (GCs) to assess their clinical application for diagnosing and monitoring diseases. METHODS: We initially investigated the appropriateness of plasma miRNA assay, and then compared plasma miRNA results with the expressions in cancer tissues from eight GC patients, and also compared plasma miRNAs between pre-and post-operative paired samples from 10 GC patients. Then, plasma miRNAs (miR-17-5p, miR-21, miR-106a, miR-106b and let-7a) were analysed in 69 GC patients and 30 healthy volunteers in total. RESULTS: The initial analysis showed that miRNAs were stable and detectable in all plasma samples, and the plasma miRNA levels reflected the tumour miRNAs in most cases. The levels of these miRNAs were significantly reduced in post-operative samples. In large-scale analysis, the plasma concentrations of miRNAs (miR-17-5p, miR-21, miR-106a, miR-106b) were significantly higher in GC patients than controls (P ¼ 0.05, 0.006, 0.008 and o0.001 respectively), whereas let-7a was lower in GC patients (P ¼ 0.002). The values of the area under the receiver-operating characteristic curve were 0.721 for the miR-106b assay and 0.879 for the miR-106a/ let-7a ratio assay. CONCLUSION: Detection of circulating miRNAs might provide new complementary tumour markers for GC.

.[1] This paper compares results from a whole atmosphere-ionosphere coupled model, GAIA, with the COSMIC and TIMED/SABER observations during the 2008/2009 northern winter season. The GAIA model has assimilated meteorological reanalysis data by a nudging method. The comparison shows general agreement in the major features from the stratosphere to the ionosphere including the growth and decay of the major stratospheric sudden warming (SSW) event in 2009. During this period, a pronounced semidiurnal variation in the F region electron density and its local-time phase shift similar to the previous observations are reproduced by the model and COSMIC observation. The model suggests that the electron density variation is caused by an enhanced semidiurnal variation in the E Â B drift, which is probably related to an amplified semidiurnal migrating tide (SW2) in the lower thermosphere. The model and TIMED/SABER observation show that the SW2 tide amplifies at low latitudes from the stratosphere to the thermosphere as well as the phase variation. Possible mechanisms for the SW2 variability in the low latitude stratosphere could be the change of its propagation condition, especially the (2, 2) mode, due to changing zonal background wind and meridional temperature gradient, and/or an enhancement of its source due to redistribution of stratospheric ozone. Present results also show a prominent long-term variation of the terdiurnal migrating component (TW3) in the ionosphere and atmosphere.

Pulsating auroras show quasi-periodic intensity modulations caused by the precipitation of energetic electrons of the order of tens of keV. It is expected theoretically that not only these electrons but also subrelativistic/relativistic electrons precipitate simultaneously into the ionosphere owing to whistler mode wave-particle interactions. The height-resolved electron density profile was observed with the European Incoherent Scatter (EISCAT) Tromsø VHF radar on 17 November 2012. Electron density enhancements were clearly identified at altitudes >68 km in association with the pulsating aurora, suggesting precipitation of electrons with a broadband energy range from~10 keV up to at least 200 keV. The riometer and network of subionospheric radio wave observations also showed the energetic electron precipitations during this period. During this period, the footprint of the Van Allen Probe-A satellite was very close to Tromsø and the satellite observed rising tone emissions of the lower band chorus (LBC) waves near the equatorial plane. Considering the observed LBC waves and electrons, we conducted a computer simulation of the wave-particle interactions. This showed simultaneous precipitation of electrons at both tens of keV and a few hundred keV, which is consistent with the energy spectrum estimated by the inversion method using the EISCAT observations. This result revealed that electrons with a wide energy range simultaneously precipitate into the ionosphere in association with the pulsating aurora, providing the evidence that pulsating auroras are caused by whistler chorus waves. We suggest that scattering by propagating whistler simultaneously causes both the precipitations of subrelativistic electrons and the pulsating aurora.

Background:Several recent studies demonstrated that microRNAs (miRNAs) are stably detectable in plasma/serum. We hypothesised that plasma miRNAs concentrations contributed to potential biomarkers in patients with oesophageal squamous cell carcinoma (ESCC).Methods:We selected three oncogenic miRNAs (miR-21, miR-184, miR-221) and one tumour suppressive miRNA (miR-375), which are frequently reported in squamous cell carcinoma, as candidate targets for this plasma miRNA assay. This study was divided into three steps: (1) Determination of appropriate plasma miRNAs in preliminary tests. (2) Evaluation of whether the plasma miRNA assays could monitor tumour dynamics. (3) Validation study on the clinical application of plasma miRNA assays in 50 ESCC patients and 20 healthy volunteers.Results:(1) In preliminary tests, the plasma level of miR-21 was significantly higher (P=0.0218) and that of miR-375 (P=0.0052) was significantly lower in ESCC patients than controls. (2) The high plasma miR-21 levels reflected tumour levels in all cases (100%). The plasma level of miR-21 was significantly reduced in postoperative samples (P=0.0058). (3) On validation analysis, the plasma level of miR-21 tended to be higher in ESCC patients (P=0.0649), while that of miR-375 was significantly lower (P<0.0001) and the miR-21/miR-375 ratio was significantly higher (P<0.0001) in ESCC patients than in controls. The value of the area under the receiver-operating characteristic curve (AUC) was 0.816 for the miR-21/miR-375 ratio assay. Patients with a high plasma level of miR-21 tended to have greater vascular invasion (P=0.1554) and to show a high correlation with recurrence (P=0.0164).Conclusion:Detection of circulating miRNAs might provide new complementary tumour markers for ESCC.

The present study compares simulations of the 2009 sudden stratospheric warming (SSW) from four different whole atmosphere models. The models included in the comparison are the Ground-to-topside model of Atmosphere and Ionosphere for Aeronomy, Hamburg Model of the Neutral and Ionized Atmosphere, Whole Atmosphere Model, and Whole Atmosphere Community Climate Model Extended version (WACCM-X). The comparison focuses on the zonal mean, planetary wave, and tidal variability in the middle and upper atmosphere during the 2009 SSW. The model simulations are constrained in the lower atmosphere, and the simulated zonal mean and planetary wave variability is thus similar up to approximate to 1 hPa (50 km). With the exception of WACCM-X, which is constrained up to 0.002 hPa (92 km), the models are unconstrained at higher altitudes leading to considerable divergence among the model simulations in the mesosphere and thermosphere. We attribute the differences at higher altitudes to be primarily due to different gravity wave drag parameterizations. In the mesosphere and lower thermosphere, we find both similarities and differences among the model simulated migrating and nonmigrating tides. The migrating diurnal tide (DW1) is similar in all of the model simulations. The model simulations reveal similar temporal evolution of the amplitude and phase of the migrating semidiurnal tide (SW2); however, the absolute SW2 amplitudes are significantly different. Through comparison of the zonal mean, planetary wave, and tidal variability during the 2009 SSW, the results of the present study provide insight into aspects of the middle and upper atmosphere variability that are considered to be robust features, as well as aspects that should be considered with significant uncertainty