Experiments were performed to evaluate the hypothesis that ACE (angiotensin-converting enzyme) inhibition (enalapril) suppresses 3-NT (3-nitrotyrosine) production in the renal cortex during the early stage of Type 1 DM (diabetes mellitus) in the rat. Enalapril was administered chronically for 2 weeks to subsets of STZ (streptozotocin)-induced DM and vehicle-treated sham rats. O2− (superoxide anion) and NOx (nitrate+nitrite) levels were measured in the media bathing renal cortical slices after 90 min incubation in vitro. SOD (superoxide dismutase) activity and 3-NT content were measured in the renal cortex homogenate. Renal cortical nitrated protein was identified by proteomic analysis. Renal cortical production of O2− and 3-NT was increased in DM rats; however, enalapril suppressed these changes. DM rats also exhibited elevated renal cortical NOx production and SOD activity, and these changes were magnified by enalapril treatment. 2-DE (two-dimensional gel electrophoresis)-based Western blotting revealed more than 20 spots with positive 3-NT immunoreactivity in the renal cortex of DM rats. Enalapril treatment blunted the DM-induced increase in tyrosine nitration of three proteins ACO2, GDH1 and MMSDH (aconitase 2, glutamate dehydrogenase 1 and methylmalonate-semialdehyde dehydrogenase), each of which resides in mitochondria. These data are consistent with enalapril preventing DM-induced tyrosine nitration of mitochondrial proteins by a mechanism involving suppression of oxidant production and enhancement of antioxidant capacity, including SOD activation.

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The characteristics of sound propagation and speech transmission along a tunnel with a "T" intersection were investigated. At receivers within sight of the sound source, low frequencies were mainly attenuated around the intersection than high frequencies. At receivers out of sight of the source, high frequencies were extensively attenuated. The overall pattern of sound attenuation along the different sections of tunnel, which was calculated by the conical beam method, agreed well with the measurements in this study. Numerical calculations of reflected and diffracted waves with minimum transmission paths in a two-dimensional plane showed that reflected waves were the primary contributors to sound fields out of sight of the source. The articulation scores measured at receivers within sight of the source were high, and most of the confusion concerned syllables that could easily be misheard, even if there were a high signal-to-noise ratio. The types of syllable confusions observed at the receivers out of sight of the source appeared to have been caused by the greater deterioration in speech signals along this part of the tunnel, especially at high frequencies. The evaluation by rapid speech transmission indices (RASTI) appeared to be overestimated at the receivers out of sight of the source. Taking into account the early decay times of impulsive sound and the calculation procedures used in RASTI, it is concluded that speech intelligibility may not have been evaluated correctly by RASTI.

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