No abstract

No abstract

A carbonyl hemoglobin (HbCO) solution was stirred with a mixed powder of polymerizable 1,2-bis(2,4octadecadienoyl)-sn-glycero-3-phosp hocholine (DODPC), cholesterol and stearic acid ( 7 / 7 / 2 by mol). The mixture was extruded through polycarbonate membrane filters (final pore size = 0.2 p m 0). The average diameter of the resulting vesicles was 203 f 39 nm. The [Hb]l[Lipidl ratio (the weight ratio of Hb in vesicle to lipid) increased with the Hb concentration, and decreased with the NaCl concentration. A maximum [Hbll [lipid] ratio was observed at pH 6.9, which was the same as the isoelectric point of Hb. The vesicles were stabilized by y-irradiation (@CO) because the bilayer lipids bound each other to yield polyphospholipids. Denaturation of Hb by y-irradiation was not detected. These polyphospholipid vesicles encapsulating Hb were stable even against the freeze-thaw cycles and the freeze-drying procedure.

No abstract

No abstract