Accurate monitoring of treatment is crucial in minimally-invasive radiofrequency ablation in oncology and cardiovascular disease. We investigated alterations in optical properties of ex-vivo bovine tissues of the liver, heart, muscle, and brain, undergoing the treatment. Time-domain diffuse optical spectroscopy was used, which enabled us to disentangle and quantify absorption and reduced scattering spectra. In addition to the well-known global (1) decrease in absorption, and (2) increase in reduced scattering, we uncovered new features based on sensitive detection of spectral changes. These absorption spectrum features are: (3) emergence of a peak around 840 nm, (4) redshift of the 760 nm deoxyhemoglobin peak, and (5) blueshift of the 970 nm water peak. Treatment temperatures above 100 °C led to (6) increased absorption at shorter wavelengths, and (7) further decrease in reduced scattering. This optical behavior provides new insights into tissue response to thermal treatment and sets the stage for optical monitoring of radiofrequency ablation.
Radiofrequency ablation (RFA) is minimally invasive thermotherapy, where a heating source is used to target and kill malignant cells in a tissue. While RFA has tremendous potential in the field of oncology, there is also a need for reliable real-time monitoring of this procedure to avoid over or under treatment. In this work, we investigate the use of timeresolved diffuse optical spectroscopy (DOS) to continuously track the change in optical properties during RFA to monitor the process of ablation. The time evolution of the spectra of the optical properties of the tissue undergoing treatment gives deep insights into the structural and constitutional changes occurring during the RFA treatment.
This work concerns the development and testing of a setup that uses laser-induced ultrasound sources to achieve synthetic transmit aperture ultrasound imaging. The sources are created by sequentially firing 32 contiguous multi-mode optical fibers to illuminate an optically absorbing film with nanosecond-pulsed laser light. Ultrasound is generated by the photoacoustic effect and insonifies the sample under investigation. Ultrasound that has interacted with the sample is detected in reflection mode using a conventional ultrasound transducer array. We present a custom-developed optical fiber multiplexing setup that enables sequential firing of the optical fiber array and characterize the acoustic fields produced by the laser-induced approach using hydrophone measurements. The integrated setup is used to make images of wire phantoms. Following this, images are taken of a breast-mimicking phantom as well as the wrist of one of the authors. Imaging results from the new approach and from conventional ultrasound imaging are compared. The lateral and axial point-spread function values show broad agreement between the two approaches, whereas the phantom and in vivo images exhibit some differences in contrast values. This work is, to our knowledge, the first instance of laser-induced ultrasound synthetic transmit aperture imaging using a clinical ultrasound array.
Unresectable liver tumors are commonly treated with percutaneous radiofrequency ablation (RFA). However, this technique is associated with high recurrence rates due to incomplete tumor ablation. Accurate image guidance of the RFA procedure contributes to successful ablation, but currently used imaging modalities have shortcomings in device guidance and treatment monitoring. We explore the potential of using photoacoustic (PA) imaging combined with conventional ultrasound (US) imaging for real-time RFA guidance. To overcome the low penetration depth of light in tissue, we have developed an annular fiber probe (AFP), which can be inserted into tissue enabling interstitial illumination of tissue. The AFP is a cannula with 72 optical fibers that allows an RFA device to slide through its lumen, thereby enabling PA imaging for RFA device guidance and ablation monitoring. We show that the PA signal from interstitial illumination is not affected by absorber-to-surface depth compared to extracorporeal illumination. We also demonstrate successful imaging of the RFA electrodes, a blood vessel mimic, a tumor-mimicking phantom, and ablated liver tissue boundaries in ex vivo chicken and bovine liver samples. PA-assisted needle guidance revealed clear needle tip visualization, a notable improvement to current US needle guidance. Our probe shows potential for RFA device guidance and ablation detection, which potentially aids in real-time monitoring.
We investigate the use of broadband time-domain diffuse optical spectroscopy (TD-DOS) to track in real time the changes in the optical properties of biological tissue undergoing radio frequency (RF) based thermal treatment.
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