Indonesia is a maritime country that is rich in seaweed. However, seaweed fermentation into lactic acid is not yet usually. Seaweed fermentation has outstanding potential because it has the most abundant polysaccharides compared to other sources. This research aims to synthesize lactic acid by fermentation using a single culture of Lactobacillus plantarum (L. plantarum) and two substrates, namely seaweed flour and refined salt Kappa-Carrageenan (RKC). Lactic acid was analyzed by fourier-transform infrared (FT-IR) spectroscopy and its concentration was determined by gas chromatography-mass spectrometry (GC-MS). The proximate analysis showed that crude Fiber and starch levels in seaweed are 25.37% and 14.66% (w/w) and also in RKC are 16.45% and 1.07% (w/w), respectively. The highest reducing sugar was attained at H2SO4 2% (w/w), which were 51,184 mg/L in RKC and 24,824 mg/L in seaweed flour. Based on FT-IR data, lactic acid characteristic signals were found at broadband approximately 3000 - 3500 cm−1, which indicated the presence of OH band, a band at 1656 cm−1 revealed C=O stretching of carbonyl groups, and a band at 1118 cm−1 for C-O stretching of alcohol. Based on GC-MS data, the highest lactic acid production was 42,267 mg/L in RKC and 37,130 mg/L in seaweed flour. In this study, we can conclude that the efficiency of hydrolysis and fermentation of RKC was better than seaweed flour. However, the substrate concentration for optimum lactic acid production was unknown, so a more in-depth study was needed.
Coal bottom ash was included in Hazardous and Toxic Waste (LB3), which requires seriously manage not to warm the environment and humans health. LB3 management becomes an obligation for the industry which producing it and being a burden in itself due to the project of waste utilization often regarded as the high-cost investment projects or less profitable. This study aims to conduct an economic assessment of pilot-scale projects to reuse bottom ash to support the clean industry strategy. The study mixed coal bottom ash with biomass from municipalities solid waste (MSW), called bio-coal fuel. These raw materials were combined with a composition 60%:40% weight (bottom ash: biomass) to be briquette form by adding amylum as a binder. This study used the benefit-cost analysis approach to assess economic feasibility. Tree indicators used in this study were net benefit-cost ratio, payback period, and return on investment. These indicators provided the company policy to continue or stop this project. The finding study showed that the company got a payback period and the net benefit from the ninth year. The company also achieved a net B/C ratio was more than one, and ROI was 1.09 times in the ninth year. The other beneficiaries acquired by the company was included external costs, such as risks from commitment failure by third parties in coal waste management, costs rising risk of purchasing coal, and given a positive value for providing employment.
The increase in plastic production has raised concerns about the depletion of fossil fuels. In addition, the environmental problems related to improper plastic waste have become progressively concerning. Polylactic acid (PLA) is one of the most intriguing biopolymers for the fabrication of biodegradable plastic. More importantly, PLA has sharply increased interest in producing its monomer, lactic acid (LA). Considering the expensive cost of LA production, exploring the potential of refined kappa-carrageenan (RKC) as a low-cost substrate is crucial. This work aimed to study RKC hydrolysate’s effect (20g L−1) on lactic acid production. A set of parameters were evaluated, including lactic acid content by high-performance liquid chromatography (HPLC) analysis, optimum bacterial growth, and reducing sugar content. The results showed that by utilizing RKC as the substrate, the growth of Lactobacillus plantarum reached its maximum within 24 hours of fermentation. The reduced sugar content decreased from 18.8 to 0.8 g L−1 after 72 hours of fermentation. More importantly, about 18.5 g L−1 of LA was produced. In conclusion, the present findings suggest that RKC can be potentially used as an alternative substrate for lowering LA production costs.
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