This study was conducted to assess the physical, chemical properties and pathological microbial contamination in the Euphrates River in Nasiriya city, southern Iraq, and three stations were chosen in the study area of 6 km for the period from the fall of 2018 until the summer of 2019. Samples were collected during this period. The first station was north of Nasiriya governorate. The second station is located in the center of the governorate. The third is located in the south of Al-Nasiriya Governorate, about 4 km from the second station. The study included measuring some physical, chemical and microbial properties of river water. It also included measuring the concentration of some physical analyzes including color, odor, temperature and turbidity. Chemical analysis included pH, BOD5, and COD. Pathogenic bacteria analyses included Salmonella enterica, E. coli pseudomonas aeroginosa, Streptococcus pyogenes, Staphylococcus aurous, and Klebseilla spp. AST in our study revealed high levels of resistance to ampicillin (100%), nalidixic acid (90%), sulfamethoxazole- trimethoprim (80% (Tetracycline and ciprofloxacin (55%) and Chloramphenicol (45%).
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